Institute of Biochemistry, Medical Faculty in Nis, , Nis, Serbia.
Amino Acids. 2010 Jan;38(1):339-45. doi: 10.1007/s00726-009-0266-3. Epub 2009 Mar 13.
Cholestatic encephalopathy results from accumulation of unconjugated bilirubin and hydrophobic bile acids in the brain. The aim of this study was to determine disturbances of polyamine metabolism in the brains of rats with experimental extrahepatic cholestasis and the effects of L-arginine administration. Wister rats were divided into groups: I: sham-operated, II: rats treated with L-arginine, III: animals with bile-duct ligation (BDL), and IV: cholestatic-BDL rats treated with L-arginine. Increased plasma gamma-glutamyltransferase and alkaline phosphatase activity and increased bile-acids and bilirubin levels in BDL rats were reduced by administration of L-arginine (P < 0.001). Cholestasis increased the brain's putrescine (P < 0.001) and decreased spermidine and spermine concentration (P < 0.05). The activity of polyamine oxidase was increased (P < 0.001) and diamine oxidase was decreased (P < 0.001) in the brains of BDL rats. Cholestasis increased the activity of arginase (P < 0.05) and decreased the level of citrulline (P < 0.001). Administration of L-arginine in BDL rats prevents metabolic disorders of polyamines and establishes a neuroprotective role in the brain during cholestasis.
胆汁淤积性脑病是由于未结合胆红素和疏水性胆汁酸在大脑中的积累而导致的。本研究旨在确定实验性肝外胆汁淤积大鼠大脑中多胺代谢的紊乱以及精氨酸给药的影响。Wistar 大鼠分为以下几组：I：假手术组，II：精氨酸治疗组，III：胆管结扎组（BDL），IV：BDL 胆汁淤积大鼠的精氨酸治疗组。精氨酸给药降低了 BDL 大鼠的血浆γ-谷氨酰转移酶和碱性磷酸酶活性以及胆汁酸和胆红素水平（P < 0.001）。胆汁淤积增加了大脑中的腐胺（P < 0.001），降低了精脒和精胺的浓度（P < 0.05）。BDL 大鼠大脑中的多胺氧化酶活性增加（P < 0.001），二胺氧化酶活性降低（P < 0.001）。胆汁淤积增加了精氨酸酶的活性（P < 0.05）并降低了瓜氨酸的水平（P < 0.001）。在 BDL 大鼠中给予精氨酸可预防多胺代谢紊乱，并在胆汁淤积期间在大脑中发挥神经保护作用。
