p16和PTCH在黑色素瘤和基底细胞癌发病机制中的作用。

Involvement of p16 and PTCH in pathogenesis of melanoma and basal cell carcinoma.

作者信息

Cretnik Maja, Poje Gorazd, Musani Vesna, Kruslin Bozo, Ozretic Petar, Tomas Davor, Situm Mirna, Levanat Sonja

机构信息

Laboratory for Hereditary Cancer, Division of Molecular Medicine, Rudjer Boskovic Institute, 10002 Zagreb, Croatia.

出版信息

Int J Oncol. 2009 Apr;34(4):1045-50. doi: 10.3892/ijo_00000230.

DOI:10.3892/ijo_00000230

PMID:19287961

Abstract

The involvement of two tumor suppressors p16 and Ptch in pathogenesis of cutaneous melanomas and basal cell carcinomas (BCCs) was studied through expression of Ptch and p16 and genetic alterations in 9p21 region (p16) and in 9q22.3 region (PTCH) of chromosome 9. Immunohistochemical analyses of paraffin-embedded tissues with Ptch and p16 antibodies, typing for 9q22-q31 and 9p21 region with polymorphic markers and p16 and Ptch mutation detection was done. Higher expression of p16 and Ptch in melanoma and BCC of the skin was frequently detected in studied cases. However, allelic loss of PTCH region occurs more frequently in BCCs than loss of heterozygosity of p16 region. Both types of tumors, BCCs and melanomas, suggest involvement of Hh-Gli signaling pathway, but using different mechanisms.

摘要

通过检测9号染色体9p21区域（p16）和9q22.3区域（PTCH）的Ptch和p16表达及基因改变，研究了两种肿瘤抑制因子p16和Ptch在皮肤黑色素瘤和基底细胞癌（BCC）发病机制中的作用。采用Ptch和p16抗体对石蜡包埋组织进行免疫组化分析，用多态性标记对9q22 - q31和9p21区域进行分型，并检测p16和Ptch突变。在所研究的病例中，经常检测到皮肤黑色素瘤和BCC中p16和Ptch的高表达。然而，PTCH区域的等位基因缺失在BCC中比p16区域的杂合性缺失更频繁发生。BCC和黑色素瘤这两种肿瘤均提示Hh - Gli信号通路的参与，但机制不同。