Kim Yong Min, Choi Young Seok, Choi Jin Woong, Park Yong Ho, Koo Bon Seok, Roh Hwan-Jung, Rha Ki-Sang
Department of Otorhinolaryngology-Head and Neck Surgery, Chungnam National University College of Medicine, Daejeon, Korea.
Laryngoscope. 2009 May;119(5):993-9. doi: 10.1002/lary.20187.
OBJECTIVE/HYPOTHESIS: The purpose of the study was to investigate the effect of intravenous adipose tissue-derived stem cell (ADSC) transplantation on olfactory epithelium regeneration following transection of the olfactory nerve in rats.
This was a experimental study using primary cultures of mesenchymal stem cells derived from animal adipose tissue with histological analysis of animal olfactory tissue.
All rats underwent unilateral transection of the olfactory nerve to induce degeneration of olfactory epithelium, and then were observed for regeneration according to time sequences. ADSCs were cultivated from neck adipose tissue of rats, and systemically injected into the experimental group. The control group was injected with phosphate buffered solution, instead of ADSCs. After 30 days, regeneration of olfactory epithelium was observed with olfactory marker protein (OMP) and proliferating cell nuclear antigen. To observe the characteristics of the transplanted ADSCs, olfactory epithelium was stained with von Willebrant factor and OMP.
After olfactory nerve transection, mature olfactory cells disappeared in 5 days, but gradually regained their thickness with increased cell numbers at approximately 10 to 15 days. By 30 days post-transection, the thickness and cellular composition of epithelium was almost restored to baseline levels pretransection. However, OMP expressions remained decreased compared with day 0 or 3. Systemically injected ADSCs were transplanted into the olfactory epithelium and survived beyond 4 weeks. The ADSCs promoted regeneration of olfactory epithelium in the animal model and differentiated into olfactory receptor neurons and endothelial cells.
Our findings suggest the feasibility of ADSC transplantation as a treatment for head trauma-related olfactory dysfunction.
目的/假设：本研究旨在探讨静脉注射脂肪组织来源干细胞（ADSC）对大鼠嗅神经横断后嗅上皮再生的影响。
这是一项实验研究，使用动物脂肪组织来源的间充质干细胞原代培养物，并对动物嗅组织进行组织学分析。
所有大鼠均接受单侧嗅神经横断以诱导嗅上皮退变，然后按时间顺序观察其再生情况。从大鼠颈部脂肪组织中培养ADSCs，并将其全身注射到实验组。对照组注射磷酸盐缓冲溶液，而非ADSCs。30天后，用嗅觉标记蛋白（OMP）和增殖细胞核抗原观察嗅上皮的再生情况。为观察移植的ADSCs的特性，用血管性血友病因子和OMP对嗅上皮进行染色。
嗅神经横断后，成熟嗅细胞在5天内消失，但在大约10至15天时逐渐恢复厚度且细胞数量增加。横断后30天，上皮的厚度和细胞组成几乎恢复到横断前的基线水平。然而，与第0天或第3天相比，OMP表达仍降低。全身注射的ADSCs移植到嗅上皮中并存活超过4周。ADSCs促进了动物模型中嗅上皮的再生，并分化为嗅觉受体神经元和内皮细胞。
我们的研究结果表明ADSC移植作为治疗头部创伤相关嗅觉功能障碍的一种方法具有可行性。
