白细胞介素-1α和牙髓卟啉单胞菌在人成骨细胞中对核因子κB受体激活剂配体表达的上调作用。

The upregulation of receptor activator NF-kappaB ligand expression by interleukin-1alpha and Porphyromonas endodontalis in human osteoblastic cells.

作者信息

Chen S-C, Huang F-M, Lee S-S, Li M-Z, Chang Y-C

机构信息

Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.

出版信息

Int Endod J. 2009 Apr;42(4):375-80. doi: 10.1111/j.1365-2591.2008.01539.x.

DOI:10.1111/j.1365-2591.2008.01539.x

PMID:19298221

Abstract

AIM

To investigate the receptor activator of nuclear factor-kappa B (NF-kappaB) ligand (RANKL) in osteoblastic cells stimulated with inflammatory mediators.

METHODOLOGY

The expression of RANKL in human osteoblastic cell line U2OS stimulated by pro-inflammatory cytokine interleukin (IL)-1alpha and black-pigmented bacteria Porphyromonas endodontalis was investigated by Western blot and enzyme-linked immunosorbent assay (ELISA). The significance of the results obtained from control and treated groups was statistically analysed by the paired Student's t-test.

RESULTS

IL-1alpha was found to upregulate RANKL production in U2OS cells (P < 0.05). Investigations of the time dependence of RANKL expression in IL-1alpha-treated cells revealed a rapid accumulation of RANKL protein after 1 h of exposure; it remained elevated throughout the 24-h incubation period shown by Western blot and ELISA. In addition, P. endodontalis also increased RANKL expression in U2OS cells after 4-h incubation period demonstrated by Western blot and ELISA (P < 0.05).

CONCLUSIONS

IL-1alpha and P. endodontalis may be involved in developing apical periodontitis through the stimulation of RANKL production.

摘要

目的

研究炎症介质刺激下成骨细胞中核因子-κB（NF-κB）配体受体激活剂（RANKL）的情况。

方法

采用蛋白质免疫印迹法和酶联免疫吸附测定法（ELISA），研究促炎细胞因子白细胞介素（IL）-1α和产黑色素细菌牙髓卟啉单胞菌刺激人成骨细胞系U2OS后RANKL的表达情况。通过配对学生t检验对对照组和处理组所得结果的显著性进行统计学分析。

结果

发现IL-1α可上调U2OS细胞中RANKL的产生（P<0.05）。对IL-1α处理细胞中RANKL表达的时间依赖性研究表明，暴露1小时后RANKL蛋白迅速积累；蛋白质免疫印迹法和ELISA显示，在整个24小时的孵育期内其水平一直升高。此外，蛋白质免疫印迹法和ELISA显示，牙髓卟啉单胞菌在孵育4小时后也增加了U2OS细胞中RANKL的表达（P<0.05）。