通过接触角进行分子识别：DNA杂交的概念验证

Molecular recognition by contact angle: proof of concept with DNA hybridization.

作者信息

Bergese Paolo, Oliviero Giulio, Colombo Italo, Depero Laura E

机构信息

Chemistry for Technologies Laboratory and INSTM, University of Brescia, Via Branze 38, 25123 Brescia, Italy.

出版信息

Langmuir. 2009 Apr 21;25(8):4271-3. doi: 10.1021/la900428u.

DOI:10.1021/la900428u

PMID:19301878

Abstract

A molecular recognition reaction supported by a solid-phase assay drives a specific change in the solid-solution interfacial tension. This prompts contact angle (CA) analysis, being a straightforward route to evaluate this property, to play the unedited role of label-free probe of the reaction. The concept is proven by the successful recognition of DNA hybridization and is further supported by the agreement between the results and the underpinning thermodynamics.

摘要

由固相分析支持的分子识别反应驱动固液界面张力发生特定变化。这促使接触角（CA）分析——作为评估该性质的直接途径——充当反应的无标记探针这一未被编辑的角色。DNA杂交的成功识别证明了这一概念，结果与基础热力学之间的一致性进一步支持了该概念。