Department of Food Science and Technology, Faculty of Marine Science, Tokyo University of Marine Science and Technology, Tokyo, Japan.
J Appl Microbiol. 2009 Aug;107(2):485-97. doi: 10.1111/j.1365-2672.2009.04223.x. Epub 2009 Mar 16.
To elucidate the detailed mechanism of histamine production by Photobacterium damselae subsp. damselae.
Histidine decarboxylase and related genes of P. damselae subsp. damselae were cloned, and three open reading frames named as hdcT, hdcA and hisRS were identified. The hdcA gene encodes a polypeptide of 377 amino acids and is considered to be the pyridoxal-P dependent histidine decarboxylase. The hdcT gene is assumed to be a histidine/histamine antiporter, and the hisRS gene is considered to be a histidyl-tRNA synthetase. Recombinant Escherichia coli strains harbouring plasmids carrying the P. damselae hdc genes were shown to over-excrete histamine extracellularly. Northern blot analysis and quantitative RT-PCR revealed high levels of mono- and bi-cistronic transcripts of hdcA, hdcT and hisRS genes under conditions of low pH and histidine excess.
The hdcA gene of P. damselae was constructed as an operon with putative histidine/histamine antiporter and histidyl-tRNA synthetase. Mono- and poly-cistronic transcripts and acid induction were detected.
This is the first report of cloning the histidine decarboxylase gene cluster in gram-negative bacteria. Also, these genes were induced under acidic conditions and in the presence of excess histidine.
阐明美人鱼发光杆菌美人鱼亚种产生组氨酸的详细机制。
克隆了美人鱼发光杆菌美人鱼亚种的组氨酸脱羧酶和相关基因，并鉴定了三个开放阅读框，分别命名为 hdcT、hdcA 和 hisRS。hdcA 基因编码一个由 377 个氨基酸组成的多肽，被认为是依赖吡哆醛-P 的组氨酸脱羧酶。hdcT 基因被认为是组氨酸/组胺反向转运蛋白，而 hisRS 基因被认为是组氨酸-tRNA 合成酶。携带美人鱼发光杆菌 hdc 基因质粒的重组大肠杆菌菌株被证明能够在外分泌组胺。Northern blot 分析和定量 RT-PCR 显示，在低 pH 值和组氨酸过量的条件下，hdcA、hdcT 和 hisRS 基因的单顺反子和双顺反子转录本水平较高。
美人鱼发光杆菌的 hdcA 基因被构建为一个与假定的组氨酸/组胺反向转运蛋白和组氨酸-tRNA 合成酶组成的操纵子。检测到单顺反子和多顺反子转录本以及酸诱导。
这是首次克隆革兰氏阴性菌组氨酸脱羧酶基因簇的报道。此外，这些基因在酸性条件下和存在过量组氨酸时被诱导。
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