Li Kai, Zeng Shuqi, Gao Jiechun, Chen Lian, Wang Ying
Department of Pediatric Surgery, Children's Hospital of Fudan University, 399 Wanyuan Road, 201102 Shanghai, PR, China.
J Pediatr Surg. 2009 Mar;44(3):565-70. doi: 10.1016/j.jpedsurg.2008.07.008.
We established a vitamin A-deficient (VAD) model of pregnant rats to evaluate the effect of vitamin A deficiency in maternal rats on tumor formation in filial rats.
Ten pregnant Wistar rats were divided into 2 groups: (1) VAD group, 6 rats were given nonvitamin A diet from 2 weeks before mating till delivery and (2) normal diet (ND) group, 4 rats were given normal diet. Twenty random neonatal rats from each group were killed on the next day of delivery. The rest neonates were given normal diet for 1 year until killed. Serum levels of vitamin A, morphology of the kidney, incidence of tumor formation, and retinoid X receptor (RXR) alpha messenger RNA (mRNA) expression in renal tissue were assessed for the filial rats.
Fifty-six and 49 neonatal rats were born for VAD group and ND group, respectively. The detection rate of nephrogenic rests (NRs) from neonates in VAD group (50%) was significantly higher than that in ND group (20%; P < .05). The incidence of nephroblastoma was 13.9% in filial rats of VAD group and 0% for ND group. The detection rate of NRs for filial rats of VAD group (30.6%) was significantly higher than that of ND group (6.9%; P < .01). The expression of RXRalpha mRNA in tumor tissue of the filial rats of VAD group (3.17 +/- 0.15) was significantly lower than that in kidney tissue of ND group (3.58 +/- 0.20; P < .01).
Deficiency in vitamin A for pregnant rats resulted in renal dysplasia, increased NRs, and higher incidence of nephroblastoma.
我们建立了孕鼠维生素A缺乏(VAD)模型,以评估母鼠维生素A缺乏对仔鼠肿瘤形成的影响。
将10只孕Wistar大鼠分为2组:(1)VAD组,6只大鼠从交配前2周直至分娩给予无维生素A饮食;(2)正常饮食(ND)组,4只大鼠给予正常饮食。每组20只随机选取的新生大鼠在分娩次日处死。其余新生大鼠给予正常饮食1年直至处死。对仔鼠评估血清维生素A水平﹑肾脏形态﹑肿瘤形成发生率以及肾组织中视黄醇X受体(RXR)α信使核糖核酸(mRNA)表达。
VAD组和ND组分别出生56只和49只新生大鼠。VAD组新生大鼠肾源性残留(NRs)检出率(50%)显著高于ND组(20%;P <.05)。VAD组仔鼠肾母细胞瘤发生率为13.9%,ND组为0%。VAD组仔鼠NRs检出率(30.6%)显著高于ND组(6.9%;P <.01)。VAD组仔鼠肿瘤组织中RXRα mRNA表达(3.17±0.15)显著低于ND组肾组织(3.58±0.20;P <.01)。
孕鼠维生素A缺乏导致肾脏发育异常、NRs增加以及肾母细胞瘤发生率升高。