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母鼠维生素A缺乏对仔鼠肿瘤形成的影响。

The effect of vitamin A deficiency in maternal rats on tumor formation in filial rats.

作者信息

Li Kai, Zeng Shuqi, Gao Jiechun, Chen Lian, Wang Ying

机构信息

Department of Pediatric Surgery, Children's Hospital of Fudan University, 399 Wanyuan Road, 201102 Shanghai, PR, China.

出版信息

J Pediatr Surg. 2009 Mar;44(3):565-70. doi: 10.1016/j.jpedsurg.2008.07.008.

Abstract

PURPOSE

We established a vitamin A-deficient (VAD) model of pregnant rats to evaluate the effect of vitamin A deficiency in maternal rats on tumor formation in filial rats.

METHODS

Ten pregnant Wistar rats were divided into 2 groups: (1) VAD group, 6 rats were given nonvitamin A diet from 2 weeks before mating till delivery and (2) normal diet (ND) group, 4 rats were given normal diet. Twenty random neonatal rats from each group were killed on the next day of delivery. The rest neonates were given normal diet for 1 year until killed. Serum levels of vitamin A, morphology of the kidney, incidence of tumor formation, and retinoid X receptor (RXR) alpha messenger RNA (mRNA) expression in renal tissue were assessed for the filial rats.

RESULTS

Fifty-six and 49 neonatal rats were born for VAD group and ND group, respectively. The detection rate of nephrogenic rests (NRs) from neonates in VAD group (50%) was significantly higher than that in ND group (20%; P < .05). The incidence of nephroblastoma was 13.9% in filial rats of VAD group and 0% for ND group. The detection rate of NRs for filial rats of VAD group (30.6%) was significantly higher than that of ND group (6.9%; P < .01). The expression of RXRalpha mRNA in tumor tissue of the filial rats of VAD group (3.17 +/- 0.15) was significantly lower than that in kidney tissue of ND group (3.58 +/- 0.20; P < .01).

CONCLUSION

Deficiency in vitamin A for pregnant rats resulted in renal dysplasia, increased NRs, and higher incidence of nephroblastoma.

摘要

目的

我们建立了孕鼠维生素A缺乏(VAD)模型,以评估母鼠维生素A缺乏对仔鼠肿瘤形成的影响。

方法

将10只孕Wistar大鼠分为2组:(1)VAD组,6只大鼠从交配前2周直至分娩给予无维生素A饮食;(2)正常饮食(ND)组,4只大鼠给予正常饮食。每组20只随机选取的新生大鼠在分娩次日处死。其余新生大鼠给予正常饮食1年直至处死。对仔鼠评估血清维生素A水平﹑肾脏形态﹑肿瘤形成发生率以及肾组织中视黄醇X受体(RXR)α信使核糖核酸(mRNA)表达。

结果

VAD组和ND组分别出生56只和49只新生大鼠。VAD组新生大鼠肾源性残留(NRs)检出率(50%)显著高于ND组(20%;P <.05)。VAD组仔鼠肾母细胞瘤发生率为13.9%,ND组为0%。VAD组仔鼠NRs检出率(30.6%)显著高于ND组(6.9%;P <.01)。VAD组仔鼠肿瘤组织中RXRα mRNA表达(3.17±0.15)显著低于ND组肾组织(3.58±0.20;P <.01)。

结论

孕鼠维生素A缺乏导致肾脏发育异常、NRs增加以及肾母细胞瘤发生率升高。

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