[Caco-2细胞体外吸收模型的建立与评价]
[Establishment and assessment of Caco-2 cell in vitro absorption model].
作者信息
Zha Long-Ying, Luo Hai-Ji, Deng Hong, Chu Xin-Wei
机构信息
Department of Nutrition and Food Hygiene, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2009 Mar;29(3):548-50.
OBJECTIVE
To establish and assess the Caco-2 cell in vitro absorption model.
METHODS
Caco-2 cells were cultured on the millipore filters fixed in Snapwell transport chamber. The cell morphology, transepithelial electrical resistance, mannitol efflux rate and alkaline phosphatase activities were monitored during culture.
RESULTS
After 21 days of in vitro culture, formation of tight junction was observed between the cells. The transepithelial electrical resistance reach a relatively stable value of 620-/+47 Omega.cm(2), the mannitol efflux rate was lower than 0.3%.h(-1).cm(-2), and the alkaline phosphatase activity in the apical side was significantly higher than that in the basolateral side.
CONCLUSION
The established Caco-2 cell model shows similar morphology to intestinal epithelial cells with formation of polarity, and can be used as an in vitro model for absorption studies.
目的
建立并评估Caco-2细胞体外吸收模型。
方法
将Caco-2细胞培养在固定于Snapwell转运小室的微孔滤膜上。在培养过程中监测细胞形态、跨上皮电阻、甘露醇外排率和碱性磷酸酶活性。
结果
体外培养21天后，观察到细胞间紧密连接的形成。跨上皮电阻达到相对稳定的值620±47Ω·cm²，甘露醇外排率低于0.3%·h⁻¹·cm⁻²，且顶端侧碱性磷酸酶活性显著高于基底外侧侧。
结论
所建立的Caco-2细胞模型显示出与肠上皮细胞相似的形态且具有极性形成，可作为吸收研究的体外模型。
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