Chen Yiting, Jiang Yingyan, Lin Zhenyu, Sun Jianjun, Zhang Lan, Chen Guonan
Ministry of Education Key Laboratory of Analysis and Detection for Food Safety (Fuzhou University), Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China.
Analyst. 2009 Apr;134(4):731-7. doi: 10.1039/b811630j. Epub 2009 Jan 16.
An electrochemiluminescence (ECL) detection system equipped with an electrically heated indium-tin-oxide (ITO) electrode was constructed. The heated ITO electrode (HITOE) coupled the advantages of a heated electrode with the optical transparency property of ITO glass. Compared with the conventional heated electrode, the HITOE is integrated into the ECL cell, which could save the expensive luminescent reagent. The temperature of the electrode (T(e)) could be accurately controlled by electrically heating. The Ru(bpy)(3)(2+)/TPrA ECL system and the colchicine/Ru(bpy)(3)(2+) ECL system were used to evaluate the developed ECL detection system. The higher sensitivity and lower RSD of ECL detection for tri-n-propylamine (TPrA) and colchicine were gained by moderately heating the electrode. The proposed method has been successfully utilized to the assay of colchicine in a spiked human serum sample. The results indicated that the developed ECL detection system could provide high sensitivity and excellent reproducibility for analytical practice.
构建了一种配备电加热铟锡氧化物（ITO）电极的电化学发光（ECL）检测系统。加热的ITO电极（HITOE）兼具加热电极的优点和ITO玻璃的光学透明特性。与传统加热电极相比，HITOE集成于ECL池中，可节省昂贵的发光试剂。电极温度（T(e)）可通过电加热精确控制。采用Ru(bpy)(3)(2+)/TPrA ECL体系和秋水仙碱/Ru(bpy)(3)(2+) ECL体系对所开发的ECL检测系统进行评估。通过适度加热电极，获得了对三正丙胺（TPrA）和秋水仙碱更高的ECL检测灵敏度和更低的相对标准偏差（RSD）。所提出的方法已成功应用于加标人血清样品中秋水仙碱的测定。结果表明，所开发的ECL检测系统可为分析实践提供高灵敏度和出色的重现性。
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