[构建用于好氧生产琥珀酸的工程大肠杆菌]

[Construction of engineered Escherichia coli for aerobic succinate production].

作者信息

Kang Zhen, Geng Yanping, Zhang Yuanyuan, Qi Qingsheng

机构信息

State Key Lab of Microbial Technology, Shandong University, Jinan 250100, China.

出版信息

Sheng Wu Gong Cheng Xue Bao. 2008 Dec;24(12):2081-5.

PMID:19306579

Abstract

Based on carbon metabolic pathway analysis of Escherichia coli MG1655, an aerobic succinate fermentation platform was constructed by knocking out five genes (ptsG, poxB, pta, iclR and sdhA), which was named E. coli QZ1111. Flask cultivation results showed that E. coli QZ1111 could accumulate succinate with a concentration of 26.4 g/L under aerobic conditions. The byproduct acetate was only 2.3 g/L. The production ratio of succinate and acetate reached 11.5:1.

摘要

基于大肠杆菌MG1655的碳代谢途径分析，通过敲除五个基因（ptsG、poxB、pta、iclR和sdhA）构建了一个好氧琥珀酸发酵平台，命名为大肠杆菌QZ1111。摇瓶培养结果表明，大肠杆菌QZ1111在好氧条件下能够积累浓度为26.4 g/L的琥珀酸。副产物乙酸仅为2.3 g/L。琥珀酸与乙酸的产量比达到11.5:1。