小鼠中沉默调节蛋白4基因（Sirt4）的荧光原位杂交及染色体组织

Fluorescence in situ hybridization and chromosomal organization of the sirtuin 4 gene (Sirt4) in the mouse.

作者信息

Mahlknecht Ulrich, Voelter-Mahlknecht Susanne

机构信息

Saarland University Medical Center, Department of Internal Medicine, Division of Immunotherapy and Gene Therapy, José Carreras Research Center, Homburg/Saar, Germany.

出版信息

Biochem Biophys Res Commun. 2009 May 15;382(4):685-90. doi: 10.1016/j.bbrc.2009.03.092. Epub 2009 Mar 21.

DOI:10.1016/j.bbrc.2009.03.092

PMID:19306844

Abstract

The sirtuins (SIRT1-7), also being referred to as class III HDACs, exert NAD-dependent deacetylase and/or ADP-ribosyltransferase activities in various cellular compartments including the cell nucleus, the cytoplasm and the mitochondria. The sirtuins play a central role in epigenetic gene silencing, DNA repair and recombination, cell-cycle, microtubule organization, and in the regulation of aging. SIRT4 is a mitochondrial protein that lacks deacetylase activities but efficiently works as an ADP-ribosyltransferase. We have isolated and characterized the murine Sirt4 genomic sequence, which spans a region of 12kb and which has one single genomic locus. Determination of the exon-intron splice junctions established that SIRT4 is encoded by 6 exons. The 1648bp murine Sirt4 transcript encodes a 418 aa protein with a predictive molecular weight of 47.3kDa. Fluorescence in situ hybridization analysis identified a single genomic locus for murine Sirt4 gene on chromosome 5F and is neighbored by the PLA2G1B and PXN genes.

摘要

沉默调节蛋白（SIRT1 - 7），也被称为Ⅲ类组蛋白去乙酰化酶，在包括细胞核、细胞质和线粒体在内的各种细胞区室中发挥依赖烟酰胺腺嘌呤二核苷酸（NAD）的去乙酰化酶和/或ADP核糖基转移酶活性。沉默调节蛋白在表观遗传基因沉默、DNA修复与重组、细胞周期、微管组织以及衰老调节中起核心作用。SIRT4是一种线粒体蛋白，缺乏去乙酰化酶活性，但能有效地作为ADP核糖基转移酶发挥作用。我们已经分离并鉴定了小鼠Sirt4基因组序列，该序列跨度为12kb，且只有一个单一的基因组位点。外显子 - 内含子剪接接头的确定表明SIRT4由6个外显子编码。1648bp的小鼠Sirt4转录本编码一个418个氨基酸的蛋白质，预测分子量为47.3kDa。荧光原位杂交分析确定小鼠Sirt4基因在5F染色体上有一个单一的基因组位点，并且与PLA2G1B和PXN基因相邻。