Yang Yanhong, Cai Shaoxi, Zheng Yimin, Lu Xiumin, Xu Xiuying, Han Yumei
Bioengineering, Chongqing University, Chongqing, China.
Curr Microbiol. 2009 Jun;58(6):635-41. doi: 10.1007/s00284-009-9382-2. Epub 2009 Mar 25.
Previously, a new Cordyceps species was isolated from a low altitude area in Chongqing, China, and named Cordyceps chongqingensis sp. nov. In this study, its anamorph was isolated and designated CQM1T. It grew optimally on Czapek medium supplied with 0.5% silkworm flour and 0.5% soybean oil meal at 25 degrees C, pH 5.0-5.5. The phenotypic and molecular characteristics were investigated for its identification and typing. Morphological observations under a microscope revealed that this anamorph of Cordyceps chongqingensis sp. nov. was a new species of Metarhizium. Moreover, it was identified as one of the variants of Metarhizium taii based on sequences of 26S rDNA D1/D2 and ITS regions, and thus named Metarhizium taii var. chongqingensis nov.
此前,从中国重庆的一个低海拔地区分离出一种新的虫草物种,并命名为重庆虫草(Cordyceps chongqingensis sp. nov.)。在本研究中,分离出了它的无性型并命名为CQM1T。它在添加0.5%蚕粉和0.5%豆粕的查氏培养基上,于25℃、pH 5.0 - 5.5条件下生长最佳。对其表型和分子特征进行了研究以进行鉴定和分型。显微镜下的形态观察表明,重庆虫草的这种无性型是绿僵菌的一个新物种。此外,根据26S rDNA D1/D2和ITS区域的序列,它被鉴定为大孢绿僵菌(Metarhizium taii)的一个变种,因此命名为大孢绿僵菌重庆变种(Metarhizium taii var. chongqingensis nov.)