Istituto Superiore di Sanità, Dipartimento del Farmaco, Rome, Italy.
Chirality. 2010 Jan;22(1):56-62. doi: 10.1002/chir.20705.
A direct semipreparative HPLC enantioseparation of two chiral thiazolidinone derivatives having cyclooxygenase-2 inhibition activity was performed on the Chiralpak IA chiral stationary phase. Semipreparative amounts of enantiopure forms were collected using acetonitrile-ethanol-trifluoroacetic acid mixtures as mobile phase. The absolute configuration of both compounds was unequivocally established by single-crystal X-ray diffraction method and correlated to the chiroptical properties of isolated enantiomers.
采用 Chiralpak IA 手性固定相，通过直接半制备高效液相色谱法对两种具有环氧化酶-2 抑制活性的手性噻唑烷酮衍生物进行对映体拆分。使用乙腈-乙醇-三氟乙酸混合物作为流动相，从半制备量的对映纯形式中收集。通过单晶 X 射线衍射方法明确确定了两种化合物的绝对构型，并将其与分离的对映异构体的手性光学性质相关联。
