在磷酸肽微阵列中对蛋白酪氨酸磷酸酶（PTPs）催化失活突变体进行高通量筛选。
High-throughput screening of catalytically inactive mutants of protein tyrosine phosphatases (PTPs) in a phosphopeptide microarray.
作者信息
Sun Hongyan, Tan Lay Pheng, Gao Liqian, Yao Shao Q
机构信息
Department of Chemistry, National University of Singapore, 3 Science Drive, Singapore 11754.
出版信息
Chem Commun (Camb). 2009 Feb 14(6):677-9. doi: 10.1039/b817853d. Epub 2008 Dec 12.
DOI:10.1039/b817853d
PMID:19322419
Abstract
We report herein a novel phosphopeptide microarray capable of noncovalently "trapping" catalytically inactive mutants of protein tyrosine phosphatases (PTPs), and its application in high-throughput determination of PTP substrate specificity.
摘要
我们在此报告一种新型磷酸肽微阵列，它能够非共价“捕获”蛋白酪氨酸磷酸酶（PTPs）的催化失活突变体，及其在高通量测定PTP底物特异性中的应用。
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