实验性自身免疫性脑脊髓炎中的机械性痛觉过敏
Mechanical hypernociception in experimental autoimmune encephalomyelitis.
作者信息
Rodrigues David Henrique, Sachs Daniela, Teixeira Antonio Lucio
机构信息
Laboratório de Imunofarmacologia, Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
出版信息
Arq Neuropsiquiatr. 2009 Mar;67(1):78-81. doi: 10.1590/s0004-282x2009000100019.
BACKGROUND
Pain is an important clinical manifestation in multiple sclerosis (MS) patients, though it has been neglected in clinical and experimental researches.
OBJECTIVE
To investigate the nociceptive response in MOG35-55 experimental autoimmune encephalomyelitis (EAE)-induced mice.
METHOD
EAE was induced in 8 to 10 week old C57BL/6 female mice with an emulsion of MOG35-55, Complete Freund Adjuvant, Mycobacterium tuberculosis H37 RA and pertussis toxin. Nociception was evaluated by the von Frey filaments method. A clinical scale ranging from 0 to 15 was used to assess motor impairment.
RESULTS
Clinical evidence of disease started at day 10 and peaked at day 14 after immunization. Thereafter, there was no worsening of symptoms until day 26. The EAE-induced mice presented reduced pressure threshold at days 7th and 10th after immunization and before the onset of clinical motor signs.
CONCLUSION
The hypernociception found validates MOG35-55 EAE as a model for the study of pain in multiple sclerosis.
背景
疼痛是多发性硬化症（MS）患者的一种重要临床表现，尽管在临床和实验研究中一直被忽视。
目的
研究髓鞘少突胶质细胞糖蛋白35-55（MOG35-55）诱导的实验性自身免疫性脑脊髓炎（EAE）小鼠的伤害性反应。
方法
用MOG35-55、完全弗氏佐剂、结核分枝杆菌H37 RA和百日咳毒素的乳剂诱导8至10周龄的C57BL/6雌性小鼠发生EAE。采用von Frey细丝法评估伤害感受。使用0至15的临床量表评估运动障碍。
结果
免疫后第10天开始出现疾病的临床证据，并在第14天达到高峰。此后，直到第26天症状都没有恶化。EAE诱导的小鼠在免疫后第7天和第10天以及临床运动体征出现之前，压力阈值降低。
结论
发现的痛觉过敏证实MOG35-55 EAE作为研究多发性硬化症疼痛的模型是有效的。
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