Hasan S H, Srivastava P, Ranjan D, Talat M
Water Pollution Research Laboratory, Department of Applied Chemistry, Institute of Technology, Banaras Hindu University, Varanasi 221005, India.
Appl Microbiol Biotechnol. 2009 Jun;83(3):567-77. doi: 10.1007/s00253-009-1984-x. Epub 2009 Mar 31.
In the present study, continuous up-flow fixed-bed column study was carried out using immobilized dead biomass of Aeromonas hydrophila for the removal of Cr(VI) from aqueous solution. Different polymeric matrices were used to immobilized biomass and polysulfone-immobilized biomass has shown to give maximum removal. The sorption capacity of immobilized biomass for the removal of Cr(VI) evaluating the breakthrough curves obtained at different flow rate and bed height. A maximum of 78.58% Cr(VI) removal was obtained at bed height of 19 cm and flow rate of 2 mL/min. Bed depth service time model provides a good description of experimental results with high correlation coefficient (> 0.996). An attempt has been made to investigate the individual as well as cumulative effect of the process variables and to optimize the process conditions for the maximum removal of chromium from water by two-level two-factor full-factorial central composite design with the help of Minitab version 15 statistical software. The predicted results are having a good agreement (R (2) = 98.19%) with the result obtained. Sorption-desorption studies revealed that polysulfone-immobilized biomass could be reused up to 11 cycles and bed was completely exhausted after 28 cycles.
在本研究中，使用嗜水气单胞菌的固定化死生物质进行连续上流式固定床柱研究，以从水溶液中去除六价铬。使用不同的聚合物基质固定生物质，结果表明聚砜固定化生物质的去除效果最佳。通过评估在不同流速和床层高度下获得的穿透曲线，研究了固定化生物质对六价铬的吸附容量。在床层高度为19 cm、流速为2 mL/min时，六价铬的最大去除率为78.58%。床层深度服务时间模型对实验结果有很好的描述，相关系数较高（> 0.996）。借助Minitab 15版统计软件，采用二级二因素全因子中心复合设计，尝试研究工艺变量的单独及累积效应，并优化工艺条件以实现从水中最大程度去除铬。预测结果与所得结果具有良好的一致性（R (2) = 98.19%）。吸附-解吸研究表明，聚砜固定化生物质可重复使用多达11个循环，28个循环后床层完全耗尽。
