用于鉴定淋病奈瑟菌多重耐药性的一步法聚合酶链反应

One-step PCR for the identification of multiple antimicrobial resistance in Neisseria gonorrhoeae.

作者信息

Lawung Ratana, Cherdtrakulkiat Rungrot, Charoenwatanachokchai Angkana, Nabu Sunanta, Suksaluk Wanvisa, Prachayasittikul Virapong

机构信息

Department of Clinical Microbiology, Faculty of Medical Technology, Mahidol University, Bangkok 10700, Thailand.

出版信息

J Microbiol Methods. 2009 Jun;77(3):323-5. doi: 10.1016/j.mimet.2009.03.009. Epub 2009 Mar 31.

DOI:10.1016/j.mimet.2009.03.009

PMID:19341768

Abstract

One-step multiplex PCR was developed for the identification of gonococci and antimicrobial-resistant profiles. From forty Neisseria gonorrhoeae isolates, the penicillinase-producing N. gonorrhoeae (PPNG), the high-level tetracycline-resistant N. gonorrhoeae (TRNG), and the ciprofloxacin-resistant N. gonorrhoeae (CRNG) were successfully classified. Our method provides expediency and benefit to epidemiology and antimicrobial-resistance mobility with 100% sensitivity and specificity for gonococcal-detection. The detection limit was 500 CFU/reaction.

摘要

开发了一步多重PCR用于淋病奈瑟菌鉴定及抗菌药物耐药谱分析。从40株淋病奈瑟菌分离株中，成功鉴定出产青霉素酶淋病奈瑟菌（PPNG）、耐高剂量四环素淋病奈瑟菌（TRNG）和耐环丙沙星淋病奈瑟菌（CRNG）。我们的方法为流行病学和抗菌药物耐药性动态分析提供了便利，淋病奈瑟菌检测的灵敏度和特异性均为100%。检测限为500 CFU/反应。