担子菌香菇的白领-2同源物PHRB是假定蓝光光感受器PHRA的一个伴侣，它与香菇酪氨酸酶基因启动子区域的一个特定位点结合。
The basidiomycetous mushroom Lentinula edodes white collar-2 homolog PHRB, a partner of putative blue-light photoreceptor PHRA, binds to a specific site in the promoter region of the L. edodes tyrosinase gene.
作者信息
Sano Hiroaki, Kaneko Shinya, Sakamoto Yuichi, Sato Toshitsugu, Shishido Kazuo
机构信息
Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori-ku, Yokohama, Japan.
出版信息
Fungal Genet Biol. 2009 Apr;46(4):333-41. doi: 10.1016/j.fgb.2009.01.001.
We isolated a cDNA homolog of Neurospora crassa wc-2 from the basidiomycetous mushroom Lentinula edodes and termed it phrB cDNA. The deduced PHRB (313 amino acid residues) contained a PAS domain and a zinc-finger motif. Random binding-site selection analysis of the PHRB produced in Escherichia coli revealed that it bound to a 7-bp sequence with the consensus sequence 5'GATA/TTG/T/AC3'. Electrophoretic mobility-shift assay showed that it also bound to the consensus sequence 5'GATATTC3' in the promoter region of the L. edodes tyrosinase gene (Le.tyr). In vitro GST-pulldown immunoblot analysis disclosed that PHRB interacts with a putative blue-light photoreceptor of L. edodes (PHRA), the homolog of N. crassa WC-1, through the PAS B- and/or PAS C domain of PHRA. The expression of phrB and Le.tyr genes in pre-primordial mycelia of L. edodes is induced by light exposure, suggesting that PHRB can regulate the expression of the Le.tyr gene in a light-dependent manner.
我们从担子菌香菇中分离出粗糙脉孢菌wc - 2的一个cDNA同源物，并将其命名为phrB cDNA。推导的PHRB（313个氨基酸残基）包含一个PAS结构域和一个锌指基序。对在大肠杆菌中产生的PHRB进行随机结合位点选择分析表明，它与一个7碱基对序列结合，其共有序列为5'GATA/TTG/T/AC3'。电泳迁移率变动分析表明，它还与香菇酪氨酸酶基因（Le.tyr）启动子区域的共有序列5'GATATTC3'结合。体外GST下拉免疫印迹分析显示，PHRB通过PHRA的PAS B和/或PAS C结构域与香菇假定的蓝光光感受器（PHRA）相互作用，PHRA是粗糙脉孢菌WC - 1的同源物。香菇原基前菌丝体中phrB和Le.tyr基因的表达受光照诱导，这表明PHRB可以以光依赖的方式调节Le.tyr基因的表达。
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