采用鸡尾酒法联合液相色谱串联质谱法评估人肝细胞中细胞色素P450的诱导作用。
Assessment of cytochrome P450 induction in human hepatocytes using the cocktail strategy plus liquid chromatography tandem mass spectrometry.
作者信息
Lahoz Agustín, Donato Maria Teresa, Picazo Laura, Castell José V, Gómez-Lechón Maria José
机构信息
Unidad Analítica Mixta Fundación, Hospital La Fe-Advancell, Valencia, Spain.
出版信息
Drug Metab Lett. 2008 Aug;2(3):205-9. doi: 10.2174/187231208785425845.
A fast and sensitive method was developed for the assessment of CYP induction in human hepatocytes cultured in 96-well plates. The effects on CYP1A2, CYP2A6, CYP2B6, CYP2C9, CYP2C19, and CYP3A4 were examined by means of mass spectrometry and the well-known cocktail strategy. The performance of the method was tested by using prototypic inducers such as methylcolanthrene, phenobarbital and rifampicin. The method was shown to be robust and predictable for the in vitro induction potential studies of drugs.
开发了一种快速灵敏的方法，用于评估在96孔板中培养的人肝细胞中的细胞色素P450（CYP）诱导情况。通过质谱法和著名的鸡尾酒策略检测了对CYP1A2、CYP2A6、CYP2B6、CYP2C9、CYP2C19和CYP3A4的影响。使用原型诱导剂如甲基胆蒽、苯巴比妥和利福平测试了该方法的性能。结果表明，该方法对于药物的体外诱导潜力研究具有稳健性和可预测性。
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