DCC 在大鼠牙胚发育中鉴别成釉细胞的表达。

Expression of DCC in differentiating ameloblasts from developing tooth germs in rats.

机构信息

Department of Oral Anatomy, School of Dentistry, Institute of Biotechnology, Wonkwang University, Iksan 570-749, South Korea.

出版信息

Arch Oral Biol. 2009 Jun;54(6):563-9. doi: 10.1016/j.archoralbio.2009.03.003. Epub 2009 Apr 11.

DOI:10.1016/j.archoralbio.2009.03.003

PMID:19362703

Abstract

OBJECTIVE

This study examined the expression pattern of the Deleted-in-colorectal-carcinoma (DCC) gene in developing rat tooth germs.

METHODS

Rat pups at 4, 7 and 10 d postpartum were used in this study. Reverse transcription-polymerase chain reaction (RT-PCR) and immunofluorescent localization were used to determine the level of DCC expression during tooth development.

RESULTS

There was more than 2-fold higher level of DCC mRNA in the rat 2nd maxillary molar tooth germs on 10 d postpartum, which was the root stage, than in the rat 3rd maxillary molar tooth germ, which was at the cap/early bell development stage. In addition, the levels of DCC mRNA in the 2nd maxillary molar germs at 4, 7 and 10 d postpartum increased gradually according to tooth development. Interestingly, immunoreactivity against DCC was specifically detected in the differentiating ameloblasts. DCC was observed in the lateral and apical sides of the newly differentiating and secretory stage ameloblasts. Afterwards, DCC was localized only in the apical side of the maturation stage ameloblasts, not in the lateral side.

CONCLUSION

DCC is expressed in the differentiating ameloblasts, which suggests that this molecule plays a crucial role in amelogenesis.

摘要

目的

本研究检测了Deleted-in-colorectal-carcinoma（DCC）基因在发育中的大鼠牙胚中的表达模式。

方法

本研究使用了产后 4、7 和 10 天的大鼠幼崽。采用逆转录-聚合酶链反应（RT-PCR）和免疫荧光定位技术来确定 DCC 在牙齿发育过程中的表达水平。

结果

在根形成期的产后第 10 天的大鼠第 2 上颌磨牙牙胚中，DCC mRNA 的水平比处于帽状/早期钟状发育阶段的大鼠第 3 上颌磨牙牙胚高 2 倍以上。此外，产后第 4、7 和 10 天的第 2 上颌磨牙牙胚中的 DCC mRNA 水平根据牙齿发育逐渐增加。有趣的是，针对 DCC 的免疫反应特异性地在分化的成釉细胞中被检测到。DCC 被观察到在新分化和分泌阶段的成釉细胞的侧面和顶端。之后，DCC 仅在成熟阶段的成釉细胞的顶端被定位，而不在侧面。