Holdaway I M, Friesen H G
Cancer Res. 1977 Jul;37(7 Pt 1):1946-52.
The specific binding of labeled porcine insulin, human prolactin, and human growth hormone was studied in 63 human breast tumors and 15 nonmalignant breast tissues. Most (90%) of the tumors demonstrated significant binding of insulin, as did 80% of nonmalignant tissues. Autoradiographic studies indicated that insulin bound dominantly to tumor cells, rather than to fat and fibrous tissue contained within tumors. Specific binding of prolactin and growth hormone of greater than 1% was seen in 20 and 12% of tumors, respectively, and one tumor studied in detail showed a small amount of saturable, high-affinity prolactin binding. The affinity of binding of insulin and prolactin to tumor was similar to that seen in target tissues in subprimate species (Kd = 4 X 10(-10) M), but the prolactin-binding capacity in the one tumor studied in detail was very low (10 fmoles/mg membrane protein), compared with prolactin-responsive experimental mammary carcinoma.
在63例人类乳腺肿瘤和15例非恶性乳腺组织中研究了标记的猪胰岛素、人催乳素和人生长激素的特异性结合。大多数（90%）肿瘤显示出胰岛素的显著结合，80%的非恶性组织也是如此。放射自显影研究表明，胰岛素主要与肿瘤细胞结合，而不是与肿瘤内的脂肪和纤维组织结合。分别在20%和12%的肿瘤中观察到催乳素和生长激素的特异性结合大于1%，对一个肿瘤进行的详细研究显示有少量可饱和的高亲和力催乳素结合。胰岛素和催乳素与肿瘤结合的亲和力与灵长类以下物种靶组织中的情况相似（解离常数Kd = 4×10⁻¹⁰ M），但与催乳素反应性实验性乳腺癌相比，在一个详细研究的肿瘤中催乳素结合能力非常低（10飞摩尔/毫克膜蛋白）。
