Barbarino-Monnier P, Gobert B, Guillet-Rosso F, Béné M C, Landes P, Faure G
Laboratoire d'Immunologie, Faculté de Médecine, Centre Hospitalier Universitaire de Nancy, France.
Fertil Steril. 1991 Nov;56(5):928-32. doi: 10.1016/s0015-0282(16)54667-6.
To determine whether the microtrauma induced by repeated puncture of ovarian follicles could result in the production of autoantibodies in women submitted to in vitro fertilization (IVF).
Antiovarian antibodies were assayed in serum samples obtained from women engaged in IVF and in serum samples from healthy controls.
Blood samples were obtained after oocyte collection in the local hospital's IVF unit.
One hundred ten women undergoing IVF entered the study. Oocyte division, numbers of embryos, and pregnancy were recorded for each woman. Control samples were from 40 individuals with no sign of autoimmune disorder.
Antiovarian antibodies were assayed in indirect immunofluorescence and enzyme-linked immunosorbent assay (ELISA) using human ovary as antigen. Specific immunoglobulin (Ig)G, IgA, and IgM were tested separately in an ELISA.
Antiovarian antibody levels were compared between patients and controls and correlated with the subsequent results of IVF.
Significantly higher levels of IgG, IgA, and IgM antiovarian antibodies were observed in the group of IVF women (P less than 0.001). IgM antiovarian antibodies correlated with a lower number of embryos (P less than 0.001) and IVF failure (P less than 0.05).
These data suggest that ovarian trauma may lead to the production of autoantibodies.
确定反复穿刺卵巢卵泡所引起的微创伤是否会导致接受体外受精（IVF）的女性产生自身抗体。
对接受IVF的女性血清样本以及健康对照者的血清样本进行抗卵巢抗体检测。
在当地医院的IVF科室采集卵母细胞后获取血样。
110名接受IVF的女性进入研究。记录每名女性的卵母细胞分裂情况、胚胎数量及妊娠情况。对照样本来自40名无自身免疫性疾病迹象的个体。
以人卵巢为抗原，采用间接免疫荧光法和酶联免疫吸附测定（ELISA）检测抗卵巢抗体。在ELISA中分别检测特异性免疫球蛋白（Ig）G、IgA和IgM。
比较患者与对照者之间的抗卵巢抗体水平，并将其与IVF的后续结果相关联。
IVF女性组中观察到IgG、IgA和IgM抗卵巢抗体水平显著更高（P<0.001）。IgM抗卵巢抗体与胚胎数量减少（P<0.001）及IVF失败（P<0.05）相关。
这些数据表明卵巢创伤可能导致自身抗体的产生。
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