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异染色质介导的基因沉默不受果蝇CBP活性的影响。

Heterochromatin-mediated gene silencing is not affected by Drosophila CBP activity.

作者信息

Smolik Sarah M

机构信息

Department of Medicine, Division of Cardiovascular Medicine L-620, Oregon Health & Sciences University, Portland, OR 97239, USA.

出版信息

J Hered. 2009 Jul-Aug;100(4):465-72. doi: 10.1093/jhered/esp016. Epub 2009 Apr 14.

Abstract

Cyclic AMP Response Element Binding protein (CREB)-binding protein (CBP) is an acetyltransferase important for modifying histones and chromatin-associated proteins and thus affecting transcription and other DNA metabolic processes. We found that the Drosophila CBP (dCBP) is associated with the NAD(+)-dependent deacetylase, SIR2, which was originally identified as a silencing information regulator in yeast that models silenced and repeated sequence chromatin such as centric heterochromatin, telomeres, and the repeated rDNA sequences. As in yeast, Drosophila sir2 (dsir2) affects the formation and/or function of centric heterochromatin. The fact that we found dCBP in immunecomplexes with dSIR2 in vivo and found that dCBP can interact with dSIR2 directly in vitro suggested that dCBP might affect the packaging of silencing heterochromatin as well. A careful study of the dCBP mutations provides evidence that dCBP does not affect the formation and/or function of centric heterochromatin and thus may affect other dSIR2 functions.

摘要

环磷腺苷反应元件结合蛋白(CREB)结合蛋白(CBP)是一种乙酰转移酶,对修饰组蛋白和染色质相关蛋白很重要,从而影响转录和其他DNA代谢过程。我们发现果蝇CBP(dCBP)与NAD(+)依赖性脱乙酰酶SIR2相关,SIR2最初被鉴定为酵母中的沉默信息调节因子,可模拟沉默和重复序列染色质,如着丝粒异染色质、端粒和重复的rDNA序列。与酵母一样,果蝇sir2(dsir2)影响着丝粒异染色质的形成和/或功能。我们在体内发现dCBP与dSIR2存在于免疫复合物中,并且在体外发现dCBP可以直接与dSIR2相互作用,这一事实表明dCBP可能也会影响沉默异染色质的包装。对dCBP突变的仔细研究提供了证据,表明dCBP不影响着丝粒异染色质的形成和/或功能,因此可能影响其他dSIR2功能。

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