Nakajima Yoshimi, Shimazawa Masamitsu, Otsubo Kazumasa, Ishibashi Takashi, Hara Hideaki
Department of Biofunctional Evaluation, Molecular Pharmacology, Gifu Pharmaceutical University, Gifu, Japan.
Curr Eye Res. 2009 Apr;34(4):311-8. doi: 10.1080/02713680902745408.
To investigate whether zeaxanthin, the predominant carotenoid pigment of the macular pigments in human retina, provides neuroprotection against retinal cell damage.
We used in vitro cultured retinal ganglion cells (RGCs), specifically RGC-5, an E1A virus-transformed rat cell line. Cell damage was induced either by a 24-hr exposure to hydrogen peroxide (H2O2) or by serum deprivation. Cell viability was measured using the tetrazolium salt, WST-8. The scavenging capacity of zeaxanthin for H2O2, superoxide anion radical (O2.-), and hydroxyl radical (HO.) was measured using a radical scavenging capacity assay with CM-H2DCFDA, a reactive oxygen species (ROS)-sensitive probe.
When added to RGC-5 cell cultures, 0.1, 10, and 1 microM zeaxanthin scavenged the free radicals induced by H2O2, O2.-, and HO., respectively. In addition, pretreatment with 1 microM zeaxanthin permitted scavenging of staurosporine-induced intracellular radicals. Zeaxanthin also inhibited the neurotoxicity induced by H2O2 or serum deprivation and scavenged the intracellular radicals induced by H2O2 or serum deprivation.
Our results suggest that zeaxanthin provides effective protection against oxidative stress-induced retinal cell damage.
研究叶黄素（人视网膜黄斑色素中的主要类胡萝卜素色素）是否能对视网膜细胞损伤提供神经保护作用。
我们使用体外培养的视网膜神经节细胞（RGCs），特别是RGC - 5，一种经E1A病毒转化的大鼠细胞系。通过24小时暴露于过氧化氢（H2O2）或血清剥夺诱导细胞损伤。使用四唑盐WST - 8测量细胞活力。使用对活性氧（ROS）敏感的探针CM - H2DCFDA通过自由基清除能力测定法测量叶黄素对H2O2、超氧阴离子自由基（O2.-）和羟基自由基（HO.）的清除能力。
当添加到RGC - 5细胞培养物中时，0.1、10和1 microM的叶黄素分别清除由H2O2、O2.-和HO.诱导的自由基。此外，用1 microM叶黄素预处理可清除星形孢菌素诱导的细胞内自由基。叶黄素还抑制由H2O2或血清剥夺诱导的神经毒性，并清除由H2O2或血清剥夺诱导的细胞内自由基。
我们的结果表明，叶黄素对氧化应激诱导的视网膜细胞损伤提供了有效的保护作用。
