Qu Zheng-hai, Xie Ning, Che Shu-yu, Lin Rong-jun, Hu Su-juan
Affiliated Hospital to College of Qingdao University, Qingdao 266003, Shandong, China.
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2009 Apr;21(4):230-3.
To investigate the effect of okam on inflammation and remodeling of airway in mice with ovalbumin (OVA) induced asthma.
Thirty-two mice of Kunming strain were divided into four groups randomly: model group, glucocorticoid inhalation group, okam group and control group, with 8 mice in each group. The asthmatic mice model was reproduced by combined injection and aerosol inhalation of OVA. The mice in model group received normal saline (0.3 ml) gavage daily. The mice in glucocorticoid inhalation group received budesonide (0.4 ml, 200 mug) and normal saline (3.6 ml) inhalation. The mice in okam group were gavaged with okam daily (50 mg/kg). The controls were given normal saline instead of OVA sensitization. All mice were sacrificed 42 days later, followed by lavage of tracheo-bronchial tree of the right lung, and the right lung was saved for pathological examination. The total cell number and differentiation in bronchoalveolar lavage fluid (BALF) were counted under microscope. The expression of interferon-gamma (IFN-gamma), interleukin-4 (IL-4) in BALF were assessed by enzyme linked immunosorbent assay (ELISA). The histological changes in the bronchi and alveoli were evaluated after hematoxylin and eosin (HE) staining. The expression of matrix metalloproteinase-9 (MMP-9) as well as the tissue inhibitor of metalloproteinase-1 (TIMP-1) were determined by immunohistochemistry.
Compared with the model group, the total cell count and IL-4 level in BALF, and the score of pathological changes in the broncho-alveolar tissue in okam group or glucocorticoid inhalation group were lower significantly, and the IFN-gamma level elevated markedly (all P<0.01). The MMP-9, TIMP-1 expression in glucocorticoid inhalation group and the TIMP-1 expression in okam group were decreased greatly (P<0.05 or P<0.01). All of above indexes showed marked differences between control group and okam group (P<0.05 or P<0.01). There were significant changes in the total cell count, IFN-gamma, pathological changes, MMP-9 and TIMP-1 between the glucocorticoid inhalation group and the okam group (P<0.05 or P<0.01).
Okam may alleviate inflammation of the bronchial and degrade the development of airway remodeling to some degree.
探讨冈田酰胺对卵清蛋白（OVA）诱导的哮喘小鼠气道炎症和重塑的影响。
将32只昆明种小鼠随机分为四组：模型组、糖皮质激素吸入组、冈田酰胺组和对照组，每组8只。采用OVA联合注射及雾化吸入法复制哮喘小鼠模型。模型组小鼠每日给予生理盐水（0.3 ml）灌胃。糖皮质激素吸入组小鼠给予布地奈德（0.4 ml，200 μg）及生理盐水（3.6 ml）雾化吸入。冈田酰胺组小鼠每日给予冈田酰胺（50 mg/kg）灌胃。对照组给予生理盐水代替OVA致敏。42天后处死所有小鼠，随后对右肺气管支气管树进行灌洗，并保存右肺用于病理检查。在显微镜下计数支气管肺泡灌洗液（BALF）中的总细胞数及细胞分化情况。采用酶联免疫吸附测定（ELISA）法检测BALF中干扰素-γ（IFN-γ）、白细胞介素-4（IL-4）的表达。苏木精-伊红（HE）染色后评估支气管和肺泡的组织学变化。采用免疫组织化学法检测基质金属蛋白酶-9（MMP-9）及金属蛋白酶组织抑制剂-1（TIMP-1）的表达。
与模型组相比，冈田酰胺组或糖皮质激素吸入组BALF中的总细胞计数及IL-4水平、支气管肺泡组织病理变化评分均显著降低，IFN-γ水平显著升高（均P<0.01）。糖皮质激素吸入组的MMP-9、TIMP-1表达及冈田酰胺组的TIMP-1表达均明显降低（P<0.05或P<0.01）。上述所有指标在对照组和冈田酰胺组之间均存在显著差异（P<0.05或P<0.01）。糖皮质激素吸入组和冈田酰胺组之间的总细胞计数、IFN-γ、病理变化、MMP-9及TIMP-1均有显著变化（P<0.05或P<0.01）。
冈田酰胺可能在一定程度上减轻支气管炎症并延缓气道重塑的发展。
