[干扰素-λ2对病毒性心肌炎小鼠心肌细胞凋亡蛋白的影响]

Nie Yu-Jun, Huang Xing-Yuan, Wang Shi-Hong

Department of Pediatrics, People's Hospital of Wuhan University, Wuhan 430060, China.

Zhongguo Dang Dai Er Ke Za Zhi. 2009 Apr;11(4):296-300.

OBJECTIVE

INF-lambda has anti-viral and anti-tumor activities. Its application in viral myocarditis (VMC) has not been reported. This study investigated the role of INF-lambda in acute VMC in mice.

METHODS

Forty mice were randomly divided into three groups: VMC (n=15), IFN-lambda2-treated VMC (n=15) and control (n=10). VMC was induced by an intraperitoneal injection of Coxsackievirus B3 (CVB3).The control group was intraperitoneally injected with 2% PBS. The IFN-lambda2-treated VMC group was administered with 400 ng IFN-lambda2 (0.1 mL) by subcutaneous injections daily, for 5 days. The control and the VMC groups were given equal amount of nomal saline.The surviving mice were sacrificed 9 days after IFN-lambda2 treatment. The pathological changes of heart tissues were examined under a light microscope. Bcl-2 and Bax expression in heart tissues was determined by immunohistochemistry.

RESULTS

The control group presented normal heart tissues. The INF-lambda2-treated VMC group showed significantly a lower pathological score (1.5+/-0.5) than the untreated VMC group (2.8+/-0.8) (P<0.01). Bcl-2 expression decreased (P<0.01), in contrast, Bax expression increased (P<0.01) in the untreated VMC group compared with that in the control group. INF-lambda2 treatment resulted in an increased Bcl-2 expression (P<0.01) and a decreased Bax expression (P<0.01) compared to the untreated VMC group.

CONCLUSIONS

INF-lambda2 may alleviate myocardial injuries and inhibit cardiomyocytic apoptosis, thus providing protective effects on myocardial cells in mice with acute VMC.

目的

干扰素-λ具有抗病毒和抗肿瘤活性。其在病毒性心肌炎（VMC）中的应用尚未见报道。本研究探讨干扰素-λ在小鼠急性VMC中的作用。

方法

40只小鼠随机分为三组：VMC组（n = 15）、干扰素-λ2治疗的VMC组（n = 15）和对照组（n = 10）。通过腹腔注射柯萨奇病毒B3（CVB3）诱导VMC。对照组腹腔注射2% PBS。干扰素-λ2治疗的VMC组每天皮下注射400 ng干扰素-λ2（0.1 mL），共5天。对照组和VMC组给予等量生理盐水。在干扰素-λ2治疗9天后处死存活小鼠。在光学显微镜下检查心脏组织的病理变化。通过免疫组织化学法测定心脏组织中Bcl-2和Bax的表达。

结果

对照组心脏组织正常。干扰素-λ2治疗的VMC组病理评分（1.5±0.5）显著低于未治疗的VMC组（2.8±0.8）（P<0.01）。与对照组相比，未治疗的VMC组Bcl-2表达降低（P<0.01），相反，Bax表达增加（P<0.01）。与未治疗的VMC组相比，干扰素-λ2治疗导致Bcl-2表达增加（P<0.01），Bax表达降低（P<0.01）。