抗绵羊κ轻链单克隆抗体。

Monoclonal antibody against sheep kappa light chain.

作者信息

Foley R C, Raison R L, Beh K J

机构信息

CSIRO Division of Animal Health, McMaster Laboratory, Glebe, NSW, Australia.

出版信息

Hybridoma. 1991 Aug;10(4):507-15. doi: 10.1089/hyb.1991.10.507.

DOI:10.1089/hyb.1991.10.507

PMID:1937501

Abstract

The production of a monoclonal antibody specific for sheep kappa light chain protein is described. The monoclonal antibody was designated McM11 and its specificity was verified using western blots of sheep IgG and slides of efferent lymph cells. The specificity of McM11 was confirmed by specific recognition of fusion proteins expressed by recombinant phage containing sheep kappa cDNAs. N terminal sequence of the light chain recognized by McM11 showed homology to kappa type light chains. McM11, together with McM6, a lambda specific monoclonal antibody, was shown by two color FACS analysis of sheep blood lymphocytes to recognize all sheep light chains.

摘要

本文描述了一种针对绵羊κ轻链蛋白的单克隆抗体的制备。该单克隆抗体命名为McM11，其特异性通过绵羊IgG的蛋白质免疫印迹法和输出淋巴细胞玻片进行了验证。通过对含有绵羊κ cDNA的重组噬菌体表达的融合蛋白的特异性识别，证实了McM11的特异性。McM11识别的轻链N端序列与κ型轻链具有同源性。通过对绵羊血液淋巴细胞的双色荧光激活细胞分选分析表明，McM11与λ特异性单克隆抗体McM6一起可识别所有绵羊轻链。