通过微阵列分析在一种新型人胰腺癌细胞模型中鉴定肝转移相关基因。
Identification of liver metastasis-related genes in a novel human pancreatic carcinoma cell model by microarray analysis.
作者信息
Shi Wei-Dong, Meng Zhi-Qiang, Chen Zhen, Lin Jun-Hua, Zhou Zhen-Hua, Liu Lu-Ming
机构信息
Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China.
出版信息
Cancer Lett. 2009 Sep 28;283(1):84-91. doi: 10.1016/j.canlet.2009.03.030. Epub 2009 Apr 17.
Pancreatic cancer with liver metastases has a poor prognosis and the molecular mechanisms remain unclear. In this study, SW1990HM, a highly metastatic human pancreatic carcinoma line was subcloned from SW1990 by intrasplenic injection. In vivo and in vitro tumorigenicity, metastatic potential, in vitro invasion, cell growth curves, plate efficiency and S-phase cell numbers were higher in SW1990HM cells. Gene expression profiles of SW1990HM and SW1990 cells showed 40 metastasis-related genes expressed with a 3-fold difference. Thirteen of these 32.5% (13/40) were adhesion and extracellular-matrix related and twelve 30% (12/40) were cell growth and proliferation related, such as MMP10, MMP9, MMP7, CDH1, MGAT5, CTNNA1, IGF1, IL8RB, ITGA7, MDM2, MET, SSTR2 and VEGF, which were related to the onset and progression of tumor metastasis. Thus, SW1990HM is an attractive model to study metastasis and identify potential therapeutic targets.
伴有肝转移的胰腺癌预后较差，其分子机制仍不清楚。在本研究中，通过脾内注射从SW1990亚克隆出高转移性人胰腺癌细胞系SW1990HM。SW1990HM细胞的体内外致瘤性、转移潜能、体外侵袭能力、细胞生长曲线、平板效率和S期细胞数量均更高。SW1990HM和SW1990细胞的基因表达谱显示，有40个与转移相关的基因表达存在3倍差异。这40个基因中的32.5%（13/40）与黏附及细胞外基质相关，30%（12/40）与细胞生长和增殖相关，如MMP10、MMP9、MMP7、CDH1、MGAT5、CTNNA1、IGF1、IL8RB、ITGA7、MDM2、MET、SSTR2和VEGF，这些基因与肿瘤转移的发生和进展有关。因此，SW1990HM是研究转移和确定潜在治疗靶点的一个有吸引力的模型。
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