Kojima Hisaki, Inagaki Minoru, Tomita Tsuyoshi, Watanabe Teruko, Uchida Satoko
Analytical Science, PreClinical Development, Banyu Pharmaceutical Co Ltd, Tsukuba, Ibaraki, Japan.
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 May 15;877(14-15):1537-42. doi: 10.1016/j.jchromb.2009.03.045. Epub 2009 Apr 1.
The O,N-deacylated derivative (deON) and polysaccharide part (PS) from the lipopolysaccharide (LPS) of Escherichia coli C strain were separated by strongly basic anion-exchange chromatography (SAX) based on the differences in the number of charged phosphate and ethanolamine substituents. They were also successfully separated and characterized by capillary zone electrophoresis and subsequent ESI-ion trap-MS (CZE/ESI-IT-MS). The O-deacylated LPS (deO) presented as a broad peak in CZE/ESI-IT-MS. However, more than twelve species could be discriminated by an extracted ion electropherogram (EIE) and monitoring the species which have different numbers of phosphate and ethanolamine substituents on polysaccharide backbone.
基于带电荷的磷酸酯和乙醇胺取代基数量的差异，通过强碱性阴离子交换色谱法（SAX）分离了大肠杆菌C菌株脂多糖（LPS）的O,N-脱酰基衍生物（deON）和多糖部分（PS）。它们也通过毛细管区带电泳和随后的电喷雾离子阱质谱（CZE/ESI-IT-MS）成功分离并进行了表征。在CZE/ESI-IT-MS中，O-脱酰基LPS（deO）呈现为一个宽峰。然而，通过提取离子电泳图（EIE）并监测多糖主链上具有不同数量磷酸酯和乙醇胺取代基的物种，可以区分出十二种以上的物种。
