使用吗啉代寡核苷酸在贾第虫中进行基因敲低
Using morpholinos for gene knockdown in Giardia intestinalis.
作者信息
Carpenter Meredith L, Cande W Zacheus
机构信息
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
出版信息
Eukaryot Cell. 2009 Jun;8(6):916-9. doi: 10.1128/EC.00041-09. Epub 2009 Apr 17.
Abstract
We used translation-blocking morpholinos to reduce protein levels in Giardia intestinalis. Twenty-four hours after electroporation with morpholinos targeting either green fluorescent protein or kinesin-2b, levels of these proteins were reduced by 60%. An epitope-tagged transgene can also be used as a reporter for morpholino efficacy with targets lacking specific antibodies.
摘要
我们使用翻译阻断吗啉代寡核苷酸来降低贾第鞭毛虫中的蛋白质水平。在用靶向绿色荧光蛋白或驱动蛋白-2b的吗啉代寡核苷酸进行电穿孔24小时后，这些蛋白质的水平降低了60%。对于缺乏特异性抗体的靶点，带有表位标签的转基因也可用作吗啉代寡核苷酸功效的报告基因。
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