Hielscher A, Eick A, Mourant J, Shen D, Freyer J, Bigio I
Opt Express. 1997 Dec 22;1(13):441-53. doi: 10.1364/oe.1.000441.
We report on the development of a method that records spatially dependent intensity patterns of polarized light that is diffusely backscattered from highly scattering media. It is demonstrated that these intensity patterns can be used to differentiate turbid media, such as polystyrene-sphere and biological-cell suspensions. Our technique employs polarized light from a He-Ne laser (l=543nm), which is focused onto the surface of the scattering medium. A surface area of approximately 4x4 cm centered on the light input point is imaged through polarization-analysis optics onto a CCD camera. One can observe a large variety of intensity patterns by varying the polarization state of the incident laser light and changing the analyzer configuration to detect different polarization components of the backscattered light. Introducing the Mueller-matrix concept for diffusely backscattered light, a framework is provided to select a subset of measurements that comprehensively describe the optical properties of backscattering media.
我们报告了一种方法的开发,该方法记录从高散射介质漫反射回来的偏振光的空间相关强度模式。结果表明,这些强度模式可用于区分浑浊介质,如聚苯乙烯球和生物细胞悬浮液。我们的技术采用来自氦氖激光器(波长 = 543nm)的偏振光,该光聚焦在散射介质的表面。以光输入点为中心的大约4×4平方厘米的表面积通过偏振分析光学器件成像到电荷耦合器件(CCD)相机上。通过改变入射激光的偏振态并改变分析仪配置以检测背向散射光的不同偏振分量,可以观察到各种各样的强度模式。引入漫反射光的穆勒矩阵概念,提供了一个框架来选择一组测量值,这些测量值全面描述了背向散射介质的光学特性。