用于特异性溶菌酶检测的阴离子共轭聚合物与染料标记的溶菌酶适体之间的荧光共振能量转移

Fluorescence resonance energy transfer between an anionic conjugated polymer and a dye-labeled lysozyme aptamer for specific lysozyme detection.

作者信息

Wang Jing, Liu Bin

机构信息

Department of Chemical & Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore.

出版信息

Chem Commun (Camb). 2009 May 7(17):2284-6. doi: 10.1039/b820001g. Epub 2009 Mar 4.

DOI:10.1039/b820001g

PMID:19377660

Abstract

Surface charge switch of a 6-carboxyfluorescein (FAM) labeled lysozyme aptamer from negative to positive upon lysozyme binding triggers fluorescence resonance energy transfer (FRET) between an anionic conjugated polymer and the aptamer, which provides a convenient method for lysozyme detection in biological media with high sensitivity and selectivity.

摘要

6-羧基荧光素（FAM）标记的溶菌酶适配体在与溶菌酶结合时，表面电荷从负变为正，触发了阴离子共轭聚合物与适配体之间的荧光共振能量转移（FRET），这为在生物介质中高灵敏度和高选择性地检测溶菌酶提供了一种便捷方法。