Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, People's Republic of China.
J Thromb Thrombolysis. 2009 Nov;28(4):444-8. doi: 10.1007/s11239-009-0333-3. Epub 2009 Apr 18.
Subtilisin QK, a new fibrinolytic enzyme, could cleave directly cross-linked fibrin in vitro. To verify the thrombolytic function of Subtilisin QK in vivo, the thrombolytic effect of purified Subtilisin QK on tail-thrombus of mouse was investigated. After injected with carrageenan, the tail-thrombus of Subtilisin QK treated group were shorter than the physiological saline treated group. Moreover, the tail-thrombus decreased correlate with Subtilisin QK in a dose-dependent manner. Thrombus nearly disappeared while the mice were treated with 12000 IU Subtilisin QK. The result indicated that Subtilisin QK significantly inhibited thrombus formation in mouse tail. This study made more foundation for further development of Subtilisin QK as a novel bifunctional thrombolytic agent.
枯草溶菌素 QK 是一种新型的纤维蛋白溶酶，能够在体外直接切割交联的纤维蛋白。为了验证枯草溶菌素 QK 在体内的溶栓功能，研究了纯化的枯草溶菌素 QK 对小鼠尾血栓的溶栓作用。在注射角叉菜胶后，枯草溶菌素 QK 处理组的尾血栓比生理盐水处理组短。此外，尾血栓的减少与枯草溶菌素 QK 呈剂量依赖性相关。当小鼠用 12000IU 的枯草溶菌素 QK 治疗时，血栓几乎消失。结果表明，枯草溶菌素 QK 能显著抑制小鼠尾血栓的形成。这项研究为进一步开发枯草溶菌素 QK 作为新型双功能溶栓剂奠定了基础。
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