通过表面等离子体共振进行表位作图

Epitope mapping by surface plasmon resonance.

作者信息

Säfsten Pär

机构信息

Department of Systems and Applications, Biacore AB (a GE Healthcare Company), Rapsgatan 7, 754 50 Uppsala, Sweden.

出版信息

Methods Mol Biol. 2009;524:67-76. doi: 10.1007/978-1-59745-450-6_5.

DOI:10.1007/978-1-59745-450-6_5

PMID:19377937

Abstract

Biacore systems (Biacore AB) enable label-free detection of molecular interactions in real time using surface plasmon resonance detection. Epitope mapping of antibodies is usually performed in a pairwise fashion where one antibody is used to capture the antigen from solution and the binding of a secondary antibody is monitored. In contrast to alternative approaches, the method allows for mapping of large matrices of antibody pairs without the need for cumbersome labeling steps, and the real-time analysis enables a better ranking of complex stability when compared with end-point assays.

摘要

Biacore系统（Biacore AB公司）利用表面等离子体共振检测技术，能够实时无标记地检测分子间相互作用。抗体的表位作图通常以成对方式进行，即使用一种抗体从溶液中捕获抗原，并监测二抗的结合情况。与其他方法相比，该方法无需繁琐的标记步骤就能对大量抗体对矩阵进行作图，而且与终点分析相比，实时分析能够更好地对复合物稳定性进行排序。