蛋白质印迹法简介。

Introduction to protein blotting.

作者信息

Kurien Biji T, Scofield R Hal

机构信息

Oklahoma Medical Research Foundation, 825 NE 13th Street, OK 73104, Oklahoma City, USA.

出版信息

Methods Mol Biol. 2009;536:9-22. doi: 10.1007/978-1-59745-542-8_3.

DOI:10.1007/978-1-59745-542-8_3

PMID:19378040

Abstract

Protein blotting is a powerful and important procedure for the immunodetection of proteins following electrophoresis, particularly proteins that are of low abundance. Since the inception of the protocol for protein transfer from an electrophoresed gel to a membrane in 1979, protein blotting has evolved greatly. The scientific community is now confronted with a variety of ways and means to carry out this transfer.

摘要

蛋白质印迹法是一种用于在电泳后对蛋白质进行免疫检测的强大且重要的方法，尤其适用于低丰度蛋白质的检测。自1979年从电泳凝胶向膜转移蛋白质的方案问世以来，蛋白质印迹法有了很大的发展。科学界目前面临着多种进行这种转移的方式和方法。