新型荧光增强 IgE 检测方法，使用 DNA 适体。
Novel fluorescence enhancement IgE assay using a DNA aptamer.
机构信息
State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
出版信息
Analyst. 2009 May;134(5):1003-7. doi: 10.1039/b812450g. Epub 2009 Mar 12.
In this paper, we demonstrate a fluorescence immunoglobulin E (IgE) assay probe based on a DNA aptamer. A Texas red-labeled short DNA strand (T-DNA) complementary with part of the IgE aptamer sequence was used to produce the fluorescence enhancement effected upon the binding of IgE to the aptamer. Another short DNA strand labeled with dabcyl quencher (Q-DNA) complementary with part of the aptamer sequence nearby the T-DNA location was used to lower the background fluorescence. The IgE can be detected in the concentration range from 9.2 x 10(-11) to 3.7 x 10(-8) mol L(-1) with a detection limit of 5.7 x 10(-11) mol L(-1).
本文展示了一种基于 DNA 适体的荧光免疫球蛋白 E（IgE）检测探针。一条与 IgE 适体序列部分互补的 Texas 红标记短 DNA 链（T-DNA）被用于产生荧光增强效应，当 IgE 与适体结合时会产生这种效应。另一条与适体序列附近 T-DNA 位置部分互补的带有二氮二苯并环辛炔（dabcyl）淬灭剂的短 DNA 链（Q-DNA）被用于降低背景荧光。该探针可以在 9.2 x 10(-11) 至 3.7 x 10(-8) mol L(-1) 的浓度范围内检测 IgE，检测限为 5.7 x 10(-11) mol L(-1)。
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