Gnjatic Sacha, Old Lloyd J, Chen Yao-Tseng
Ludwig Institute for Cancer Research, New York Branch at Memorial Sloan-Kettering Cancer Center, New York, NY, USA.
Methods Mol Biol. 2009;520:11-9. doi: 10.1007/978-1-60327-811-9_2.
With the identification of tumor antigens, the host immune response to various types of cancers can now be studied with a high degree of specificity in large cohorts of patients, in the hope of correlating immunity with clinical events and defining immunotherapeutic strategies. Several antigens, such as NY-ESO-1, p53, or SOX2, have attracted attention because of their frequent spontaneous immunogenicity in cancer patients, notably in their ability to induce humoral responses. We describe in this chapter a simple implementation of a serological monitoring platform for autoantibody measurement in cancer patients, from production of recombinant antigens to ELISA testing and interpretation.
随着肿瘤抗原的鉴定,现在可以在大量患者队列中高度特异性地研究宿主对各种癌症的免疫反应,以期将免疫与临床事件相关联并确定免疫治疗策略。几种抗原,如NY-ESO-1、p53或SOX2,因其在癌症患者中频繁出现的自发免疫原性,尤其是诱导体液反应的能力而受到关注。在本章中,我们描述了一种用于癌症患者自身抗体检测的血清学监测平台的简单实施方案,从重组抗原的制备到ELISA检测及结果解读。