Kamei Ichiro, Watanabe Masashi, Harada Kazuhisa, Miyahara Takashi, Suzuki Shinya, Matsufuji Yasushi, Kondo Ryuichiro
Department of Forest and Forest Products Sciences, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
Chemosphere. 2009 Jun;75(10):1294-300. doi: 10.1016/j.chemosphere.2009.03.028. Epub 2009 Apr 21.
To examine the bioremediation potential of Phlebia brevispora in dioxin-contaminated soil, the fungus was inoculated into autoclaved soil that was contaminated with 2,7-dichlorodibenzo-p-dioxin (2,7-DCDD) or 1,3,6,8-tetrachlorodibenzo-p-dioxin (1,3,6,8-TCDD). Three types of soils, organic-rich soil (Andosol), organic-poor soil (Granitic Regosols), and paddy soil, were used for the construction of artificially contaminated soil to understand the influence of the soil property on fungal growth and dioxin degradation ability. Under a solid-state condition, although the growth of the fungus improved in organic-rich soil, the degradation of 2,7-DCDD was inhibited. Although the degradation of 1,3,6,8-TCDD under a solid-state condition was inhibited severely, 1,3,6,8-TCDD degradation was observed under a slurry-state condition in organic-poor soil. In the case of organic-rich soil, an increase in water content improved the 1,3,6,8-TCDD degradation efficiency. When the historically contaminated paddy soil was treated with P. brevispora under a slurry-state condition, 1,3,6,8-TCDD as the main contaminant degraded 50% after 90d incubation.
为了研究短孢射脉菌对二噁英污染土壤的生物修复潜力，将该真菌接种到被2,7 - 二氯二苯并 - p - 二噁英（2,7 - DCDD）或1,3,6,8 - 四氯二苯并 - p - 二噁英（1,3,6,8 - TCDD）污染的灭菌土壤中。使用三种类型的土壤，即富含有机质的土壤（暗色土）、贫含有机质的土壤（花岗岩风化土）和水稻土，构建人工污染土壤，以了解土壤性质对真菌生长和二噁英降解能力的影响。在固态条件下，尽管该真菌在富含有机质的土壤中生长有所改善，但2,7 - DCDD的降解受到抑制。尽管在固态条件下1,3,6,8 - TCDD的降解受到严重抑制，但在贫含有机质土壤的浆态条件下观察到了1,3,6,8 - TCDD的降解。在富含有机质土壤的情况下，含水量的增加提高了1,3,6,8 - TCDD的降解效率。当在浆态条件下用短孢射脉菌处理历史污染的水稻土时，作为主要污染物的1,3,6,8 - TCDD在培养90天后降解了50%。
