桑橙（Maclura pomifera (Rafin.) Schneider）提取物及化合物的胆碱酯酶抑制作用
Cholinesterase inhibitory effects of the extracts and compounds of Maclura pomifera (Rafin.) Schneider.
作者信息
Orhan I, Senol F S, Kartal M, Dvorská M, Zemlicka M, Smejkal K, Mokrý P
机构信息
Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330 Ankara, Turkey.
出版信息
Food Chem Toxicol. 2009 Aug;47(8):1747-51. doi: 10.1016/j.fct.2009.04.023. Epub 2009 Apr 24.
We have investigated anticholinesterase potential of the methanol extracts from the leaf, wood, flower, twig, and stem bark of the female and male individuals and rhizodermis and fruit from the female tree of Maclura pomifera (Rafin.) Schneider (Moraceae) along with its major isoflavonoids; osajin and pomiferin as well as their semi-synthetic derivatives; iso-osajin and iso-pomiferin. Anticholinesterase activity was determined by Ellman method using ELISA microplate reader. Osajin and pomiferin had a noticeable inhibition of AChE with IC(50) values of 2.239 and 0.096 mM, respectively, while their iso-derivatives were found to display less inhibition towards AChE. The extracts and compounds did not inhibit BChE. The extracts were analyzed for osajin and pomiferin contents by LC-DAD-MS and only the fruits and female flowers contained osajin (fruit: 8.87%, female flowers: 0.19%, w/w) and pomiferin (fruit: 13.6%, female flowers: 0.36%, w/w).
我们研究了桑橙（Maclura pomifera (Rafin.) Schneider，桑科）雌株和雄株的叶、木、花、嫩枝、茎皮以及雌株的根皮和果实的甲醇提取物及其主要异黄酮类化合物奥沙金和波米菲林以及它们的半合成衍生物异奥沙金和异波米菲林的抗胆碱酯酶潜力。采用酶联免疫吸附测定酶标仪，通过埃尔曼法测定抗胆碱酯酶活性。奥沙金和波米菲林对乙酰胆碱酯酶有显著抑制作用，IC(50)值分别为2.239和0.096 mM，而它们的异衍生物对乙酰胆碱酯酶的抑制作用较小。提取物和化合物对丁酰胆碱酯酶没有抑制作用。通过液相色谱 - 二极管阵列 - 质谱联用仪分析提取物中的奥沙金和波米菲林含量，结果发现只有果实和雌花含有奥沙金（果实：8.87%，雌花：0.19%，w/w）和波米菲林（果实：13.6%，雌花：0.36%，w/w）。
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