2'-脱氧-1-甲基假胞苷，一种2'-脱氧-5-甲基异胞苷的稳定类似物。
2'-deoxy-1-methylpseudocytidine, a stable analog of 2'-deoxy-5-methylisocytidine.
作者信息
Kim Hyo-Joong, Leal Nicole A, Benner Steven A
机构信息
Westheimer Institute for Science and Technology, Foundation for Applied Molecular Evolution, PO Box 13174, Gainesville, FL 32604, USA.
出版信息
Bioorg Med Chem. 2009 May 15;17(10):3728-32. doi: 10.1016/j.bmc.2009.03.047. Epub 2009 Mar 27.
Abstract
2'-deoxy-5-methylisocytidine is widely used in assays to personalize the care of patients infected with HIV, hepatitis C, and other infectious agents. However, oligonucleotides that incorporate 2'-deoxy-5-methylisocytidine are expensive, because of its intrinsic chemical instability. We report here a C-glycoside analog that is more stable and, in oligonucleotides, pairs with 2'-deoxyisoguanosine, contributing to duplex stability about as much as a standard 2'-deoxycytidine and 2'-deoxyguanosine pair.
摘要
2'-脱氧-5-甲基异胞苷广泛应用于个性化护理感染艾滋病毒、丙型肝炎及其他感染因子患者的检测中。然而，由于其固有的化学不稳定性，掺入2'-脱氧-5-甲基异胞苷的寡核苷酸价格昂贵。我们在此报告一种C-糖苷类似物，它更稳定，并且在寡核苷酸中与2'-脱氧异鸟苷配对，对双链稳定性的贡献与标准的2'-脱氧胞苷和2'-脱氧鸟苷对大致相同。
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