Bakeine G J, Di Salvo M, Bortolussi S, Stella S, Bruschi P, Bertolotti A, Nano R, Clerici A, Ferrari C, Zonta C, Marchetti A, Altieri S
Department of Clinical Medicine and Neurology, Cattinara Hospital, University of Trieste, Italy.
Appl Radiat Isot. 2009 Jul;67(7-8 Suppl):S332-5. doi: 10.1016/j.apradiso.2009.03.073. Epub 2009 Mar 27.
In order for boron neutron capture therapy (BNCT) to be eligible for application in lung tumour disease, three fundamental criteria must be fulfilled: there must be selective uptake of boron in the tumour cells with respect to surrounding healthy tissue, biological effectiveness of the radiation therapy and minimal damage or collateral effects of the irradiation on the surrounding tissues. In this study, we evaluated the biological effectiveness of BNCT by in vitro irradiation of rat colon-carcinoma cells previously incubated in boron-enriched medium. One part of these cells was re-cultured in vitro while the other was inoculated via the inferior vena cava to induce pulmonary metastases in a rat model. We observed a post-irradiation in vitro cell viability of 0.05% after 8 days of cell culture. At 4 months follow-up, all animal subjects in the treatment group that received irradiated boron-containing cells were alive. No animal survived beyond 1 month in the control group that received non-treated cells (p<0.001 Kaplan-Meier). These preliminary findings strongly suggest that BNCT has a significant lethal effect on tumour cells and post irradiation surviving cells lose their malignant capabilities in vivo. This radio-therapeutic potential warrants the investigation of in vivo BNCT for lung tumour metastases.
为使硼中子俘获疗法（BNCT）有资格应用于肺部肿瘤疾病，必须满足三个基本标准：相对于周围健康组织，肿瘤细胞必须能够选择性摄取硼；放射治疗要有生物学有效性；照射对周围组织的损伤或附带影响要最小。在本研究中，我们通过对先前在富含硼的培养基中孵育的大鼠结肠癌细胞进行体外照射，评估了BNCT的生物学有效性。这些细胞的一部分在体外重新培养，而另一部分通过下腔静脉接种，以在大鼠模型中诱导肺转移。细胞培养8天后，我们观察到体外照射后细胞活力为0.05%。在4个月的随访中，接受照射含硼细胞的治疗组所有动物受试者均存活。接受未处理细胞的对照组无动物存活超过1个月（Kaplan-Meier法，p<0.001）。这些初步发现强烈表明，BNCT对肿瘤细胞有显著的致死作用，照射后存活的细胞在体内失去了恶性能力。这种放射治疗潜力值得对肺部肿瘤转移的体内BNCT进行研究。
