Sommers C H, Geveke D J, Pulsfus S, Lemmenes B
Eastern Regional Research Center, Agricultural Research Service, U.S. Dept. of Agriculture, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA.
J Food Sci. 2009 Apr;74(3):M138-41. doi: 10.1111/j.1750-3841.2009.01107.x.
Listeria monocytogenes, a psychrotrophic foodborne pathogen, is a recurring postprocess contaminant on ready-to-eat meat (RTE) products including frankfurters. Flash (Steam) Pasteurization (FP) and ultraviolet light (254 nm-UVC) has been shown to reduce levels of L. monocytogenes and L. innocua on frankfurters. In this study, the use of UVC light followed by FP to inactivate L. innocua, a nonpathogenic surrogate for L.monocytogenes, on frankfurters that contained sodium diacetate and potassium lactate (SDA/PL) in a pilot-plant setting was investigated. Application of UVC (1.0 J/cm2), followed by FP (0.75 s steam/121 degrees C) resulted in inactivation of 3.19 log L. innocua, while application of UVC (4.0 J/cm2), followed by FP (3 s steam/121 degrees C) resulted in inactivation of 3.89 log of L. innocua. A refrigerated storage study (8 degrees C) of frankfurters that contained SDA/PL that were treated with UVC followed by FP revealed the growth of L. innocua was inhibited for approximately 8 wk following application of the interventions. The use of UVC in combination with FP had little effect on frankfurter color and texture. The combination of UVC, FP, and SDA/PL was found to be an effective hurdle process for decontamination of frankfurter surfaces.
单核细胞增生李斯特菌是一种嗜冷性食源性病原体,是即食肉类(RTE)产品(包括法兰克福香肠)中反复出现的加工后污染物。闪蒸(蒸汽)巴氏杀菌(FP)和紫外线(254nm-UVC)已被证明可降低法兰克福香肠上单核细胞增生李斯特菌和无害李斯特菌的水平。在本研究中,研究了在中试工厂环境下,对含有双乙酸钠和乳酸钾(SDA/PL)的法兰克福香肠,先使用紫外线光然后进行FP处理,以灭活无害李斯特菌(单核细胞增生李斯特菌的非致病性替代菌)。施加紫外线(1.0J/cm²),随后进行FP(0.75秒蒸汽/121摄氏度)可使3.19个对数的无害李斯特菌失活,而施加紫外线(4.0J/cm²),随后进行FP(3秒蒸汽/121摄氏度)可使3.89个对数的无害李斯特菌失活。对含有SDA/PL且经紫外线处理后再进行FP处理的法兰克福香肠进行冷藏储存研究(8摄氏度),结果显示在应用这些干预措施后,无害李斯特菌的生长被抑制了约8周。紫外线与FP联合使用对法兰克福香肠的颜色和质地影响很小。发现紫外线、FP和SDA/PL的组合是一种有效的法兰克福香肠表面去污障碍工艺。