Schramm Elisabeth, Kürten Andreas, Hölzer Jasper, Mitschke Stefan, Mühlberger Fabian, Sklorz Martin, Wieser Jochen, Ulrich Andreas, Pütz Michael, Schulte-Ladbeck Rasmus, Schultze Rainer, Curtius Joachim, Borrmann Stephan, Zimmermann Ralf
Helmholtz Zentrum München, Institute of Ecological Chemistry, 85764 Neuherberg, Germany.
Anal Chem. 2009 Jun 1;81(11):4456-67. doi: 10.1021/ac900289r.
An in-house-built ion trap mass spectrometer combined with a soft ionization source has been set up and tested. As ionization source, an electron beam pumped vacuum UV (VUV) excimer lamp (EBEL) was used for single-photon ionization. It was shown that soft ionization allows the reduction of fragmentation of the target analytes and the suppression of most matrix components. Therefore, the combination of photon ionization with the tandem mass spectrometry (MS/MS) capability of an ion trap yields a powerful tool for molecular ion peak detection and identification of organic trace compounds in complex matrixes. This setup was successfully tested for two different applications. The first one is the detection of security-relevant substances like explosives, narcotics, and chemical warfare agents. One test substance from each of these groups was chosen and detected successfully with single photon ionization ion trap mass spectrometry (SPI-ITMS) MS/MS measurements. Additionally, first tests were performed, demonstrating that this method is not influenced by matrix compounds. The second field of application is the detection of process gases. Here, exhaust gas from coffee roasting was analyzed in real time, and some of its compounds were identified using MS/MS studies.
一台自行搭建的与软电离源相结合的离子阱质谱仪已安装并测试完毕。作为电离源，采用电子束泵浦真空紫外（VUV）准分子灯（EBEL）进行单光子电离。结果表明，软电离可减少目标分析物的碎片化，并抑制大多数基质成分。因此，光子电离与离子阱串联质谱（MS/MS）功能相结合，为复杂基质中有机痕量化合物的分子离子峰检测和鉴定提供了一个强大的工具。该装置已成功地针对两种不同应用进行了测试。第一个应用是检测与安全相关的物质，如爆炸物、麻醉品和化学战剂。从这些类别中各选取一种测试物质，并通过单光子电离离子阱质谱（SPI-ITMS）MS/MS测量成功检测到。此外，还进行了初步测试，证明该方法不受基质化合物的影响。第二个应用领域是过程气体的检测。在此，对咖啡烘焙产生的废气进行了实时分析，并通过MS/MS研究鉴定了其中的一些化合物。
