Pohanka Miroslav, Musilek Kamil, Kuca Kamil
Center of Advanced Studies, Faculty of Military Health Sciences, University of Defense, Trebesska 1575, Hradec Kralove, Czech Republic.
Curr Med Chem. 2009;16(14):1790-8. doi: 10.2174/092986709788186129.
Biosensors are available and applicable for detection and characterization of specific inhibitors of many enzymes. In this review, biosensors based on fixed acetylcholinesterase (AChE) or butyrylcholinesterase (BChE) are presented. Inhibition of selected enzymes by various compounds, such as organophosphorus and carbamate pesticides, nerve agents (e.g. sarin or VX), and other natural toxins (e.g. aflatoxins), was employed to develop specific assays using biosensors only. Biosensor technology brings potential miniaturization and portability, when it is compared to standard methods. Construction of biosensors based on cholinesterases became a more important issue within the last decades. Novel approach with recombinant proteins, microelectrodes and immobilization protocol related to nanotechnologies opened new insight to the cholinesterase based biosensor construction and its perspective via routine assays. This review is focused on novel trends within such biosensors as a result of the known platform.
生物传感器可用于检测和表征多种酶的特异性抑制剂。在本综述中，介绍了基于固定化乙酰胆碱酯酶（AChE）或丁酰胆碱酯酶（BChE）的生物传感器。利用各种化合物（如有机磷和氨基甲酸酯类农药、神经毒剂（如沙林或VX）以及其他天然毒素（如黄曲霉毒素））对选定酶的抑制作用，仅使用生物传感器开发了特异性检测方法。与标准方法相比，生物传感器技术具有潜在的小型化和便携性。在过去几十年中，基于胆碱酯酶的生物传感器构建成为一个更为重要的问题。与纳米技术相关的重组蛋白、微电极和固定化方案的新方法为基于胆碱酯酶的生物传感器构建及其通过常规检测的前景开辟了新的视角。本综述聚焦于基于已知平台的此类生物传感器的新趋势。
