Rubio Carlos A, Lenander Claes
Department of Pathology, Karolinska University Hospital, Stockholm, Sweden.
Anticancer Res. 2009 May;29(5):1745-8.
Studying the invading edge of colorectal carcinomas it was previously noticed that the neoplastic glands with pores could contain mucin, inflammatory cells and/or necrotic material.
To systematically record the type of intraglandular materials found in the neoplastic glands with pores at the invading edge of sporadic colonic carcinomas.
Histological sections from 12 adenocarcinomas were selected when the following materials were predominantly found in the neoplastic glands with pores at the invading tumor edge: mucin (3 cases, group I), neutrophilic granulocyes (3 cases, group II), necrotic material (3 cases, group III) and invaginated stroma (3 cases, group IV). Surgical histological sections were stained with hematoxylin and eosin (H&E) and analyzed at 20 magnifications. The length of the invading edge was measured with a translucent millimetre ruler and the number of glands with pores per mm was calculated in each case.
Two hundred and ninety-five glands with pores were analyzed in the 12 cases. Mucus was found in 68% (n=55) of the 81 glands with pores in group I, granulocytes in 95% (n=41) of the 43 glands with pores in group II, necrotic material in 72% (n=84) of the 117 glands with pores in group III and stromal invaginations in 61% (n=33) of the 54 glands with pores in group IV. The mean number of glands with pores per mm was 1.02 (55 glands/54 mm) in group I, 0.93 (41 glands/44 mm) in group II, 1.22 (84 glands /69 mm) in group III and 0.62 (33 glands /53 mm) in group IV.
Neoplastic glands with pores at the invading front of colonic adenocarcinomas may predominantly contain various biological materials. These materials may differ in different individuals. While tumor mucins harbour proteolytic enzymes able to digest the juxtaposed stroma of the host, the possible significance of the other intraglandular materials contributed by the host, such as neutrophils, necrotic material and/or stromal invaginations on tumor growth, is still poorly understood. Further studies are necessary to assess whether each one of these intraglandular materials has any bearing on the progression of colonic carcinomas.
在研究结直肠癌的浸润边缘时，此前注意到有孔的肿瘤性腺体可能含有黏液、炎性细胞和/或坏死物质。
系统记录散发性结肠癌浸润边缘有孔肿瘤性腺体中发现的腺腔内物质类型。
选取12例腺癌的组织切片，这些切片在肿瘤浸润边缘的有孔肿瘤性腺体中主要发现了以下物质：黏液（3例，I组）、中性粒细胞（3例，II组）、坏死物质（3例，III组）和内陷间质（3例，IV组）。手术组织切片用苏木精和伊红（H&E）染色，并在20倍放大倍数下进行分析。用半透明毫米尺测量浸润边缘的长度，并计算每毫米有孔腺体的数量。
对12例中的295个有孔腺体进行了分析。I组81个有孔腺体中，68%（n = 55）发现黏液；II组43个有孔腺体中，95%（n = 41）发现粒细胞；III组117个有孔腺体中，72%（n = 84）发现坏死物质；IV组54个有孔腺体中，61%（n = 33）发现间质内陷。I组每毫米有孔腺体的平均数量为1.02（55个腺体/54毫米），II组为0.93（41个腺体/44毫米），III组为1.22（84个腺体/69毫米），IV组为0.62（33个腺体/53毫米）。
结肠腺癌浸润前沿有孔的肿瘤性腺体可能主要含有各种生物物质。这些物质在不同个体中可能有所不同。虽然肿瘤黏液含有能够消化宿主相邻间质的蛋白水解酶，但宿主贡献的其他腺腔内物质，如中性粒细胞、坏死物质和/或间质内陷对肿瘤生长的可能意义仍知之甚少。需要进一步研究以评估这些腺腔内物质中的每一种是否与结肠癌的进展有关。
