Abdalla Fathi, Boder Jamela, Markus Rabia, Hashmi Hussein, Buhmeida Abdelbaset, Collan Yrjö
Department of Pathology, University of Turku, Kiinamyllynkatu 10, 20520 Turku, Finland.
Anticancer Res. 2009 May;29(5):1771-6.
The relation of nuclear morphometry measurements with clinicopathological features was evaluated along with prognosis in invasive female breast carcinoma in Libyan patients. Data was compared with corresponding results on Finnish, and Nigerian female breast cancer patients.
Histological samples from 131 patients of breast carcinoma were retrospectively studied by computerized nuclear morphometry. In each case, 50 nuclei were measured and the mean nuclear morphometric features were calculated and compared with different clinicopathological features, and patient's survival.
There was statistically significant correlation between the mean nuclear area (MNA) and most clinicopathological features, with the strongest association observed for nuclear grade (p<0.0001). There was also correlation between nuclear area and tumor stage (p<0.04), tumor size (p<0.03) and lymph node (LN) status (p<0.001). A corresponding relationship was found between other size related features and clinical factors. The univariate analysis and survival analysis indicated that short survival time was associated with high nuclear morphometric values. MNA had negative correlation with length of survival (Pearson's test r=-0.29, p=0.019). Morphometric shape features did not show significant association with clinical features or survival.
The results indicated that nuclear size features are reliable prognostic indicators in Libyan female breast carcinomas, as they were among Finnish and Nigerian females. The nuclear morphometric parameters can identify the aggressive tumor phenotype and provide significant prognostic information in predicting survival and tumors at risk of progression. The cut-off (71.0 mum(2)) of MNA might be applied as quantitative criterium for Libyan nuclear grading to separate patients into good and poor prognosis groups.
评估了利比亚浸润性女性乳腺癌患者的细胞核形态测量值与临床病理特征的关系以及预后情况。将数据与芬兰和尼日利亚女性乳腺癌患者的相应结果进行了比较。
对131例乳腺癌患者的组织学样本进行回顾性计算机化细胞核形态测量研究。在每个病例中，测量50个细胞核，并计算平均细胞核形态特征，与不同的临床病理特征及患者生存率进行比较。
平均核面积（MNA）与大多数临床病理特征之间存在统计学显著相关性，其中与核分级的相关性最强（p<0.0001）。核面积与肿瘤分期（p<0.04）、肿瘤大小（p<0.03）和淋巴结（LN）状态（p<0.001）之间也存在相关性。在其他与大小相关的特征和临床因素之间发现了相应的关系。单因素分析和生存分析表明，生存时间短与高细胞核形态测量值相关。MNA与生存长度呈负相关（Pearson检验r=-0.29，p=0.019）。形态测量形状特征与临床特征或生存率未显示出显著相关性。
结果表明，细胞核大小特征在利比亚女性乳腺癌中是可靠的预后指标，在芬兰和尼日利亚女性中也是如此。细胞核形态测量参数可以识别侵袭性肿瘤表型，并在预测生存和有进展风险的肿瘤方面提供重要的预后信息。MNA的临界值（71.0μm²）可作为利比亚细胞核分级的定量标准，将患者分为预后良好和不良组。
