Ueno H, Nakamuro K, Sayato Y, Okada S
Division of Environmental Health, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan.
Mutat Res. 1991 Nov;251(1):99-107. doi: 10.1016/0027-5107(91)90219-e.
The characteristics of mutagenesis by glyoxal in Salmonella tester strains TA100 and TA104, and particularly a possible role of active oxygen species, were investigated. Glyoxal was converted into a non-mutagenic chemical with glutathione (GSH) by glyoxalase I, and the mutagenic activity was enhanced by the depletion of intracellular GSH. Glyoxal caused the reduction of nitro blue tetrazolium, which was suppressed by the addition of 2,5-diphenylfuran, superoxide dismutase (SOD) and catalase (CAT), scavengers of singlet oxygen (1O2), superoxide radical (O2-) and hydrogen peroxide (H2O2), respectively. However, only the 1O2 scavenger almost completely suppressed the mutagenic activity of glyoxal. Mutagenicity assays using strains pretreated with N,N-diethyldithiocarbamate of a SOD inhibitor and strains with low levels of SOD and CAT indicated that the mutagenesis by glyoxal was independent of intracellular levels of SOD and CAT, though glyoxal itself repressed them. Therefore, all the results suggest that 1O2 formed from glyoxal is related to its mutagenesis, but that neither O2- nor H2O2 is intracellularly predominantly related to it. The action of glyoxal against SOD and CAT, and the formation of glyoxal adducts with amino acids as their components are also discussed.
研究了乙二醛在沙门氏菌测试菌株TA100和TA104中诱导突变的特性，特别是活性氧物种可能发挥的作用。乙二醛通过乙二醛酶I与谷胱甘肽（GSH）转化为一种非诱变化学物质，并且通过消耗细胞内GSH可增强诱变活性。乙二醛导致硝基蓝四唑还原，分别添加单重态氧（1O2）、超氧阴离子（O2-）和过氧化氢（H2O2）的清除剂2,5-二苯基呋喃、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）可抑制这种还原。然而，只有1O2清除剂几乎完全抑制了乙二醛的诱变活性。使用经SOD抑制剂N,N-二乙基二硫代氨基甲酸盐预处理的菌株以及SOD和CAT水平较低的菌株进行的诱变试验表明，尽管乙二醛本身会抑制它们，但乙二醛诱导的突变与细胞内SOD和CAT的水平无关。因此，所有结果表明，由乙二醛形成的1O2与其诱变作用有关，但O2-和H2O2在细胞内均与其主要诱变作用无关。还讨论了乙二醛对SOD和CAT的作用以及以氨基酸为成分的乙二醛加合物的形成。
