Carlsen Charlotte U, Kurtmann Lone, Brüggemann Dagmar A, Hoff Signe, Risbo Jens, Skibsted Leif H
Department of Food Science, University of Copenhagen, Frederiksberg C, Denmark.
Cryobiology. 2009 Jun;58(3):262-7. doi: 10.1016/j.cryobiol.2009.01.005.
Incorporation of the fluorescent probe C11-BODIPY(581/591) in two dried membrane systems, soy bean phosphatidylcholine liposomes freeze-dried in a carbohydrate/protein matrix and Lactobacillus acidophilus (La-5) freeze-dried in a carbohydrate matrix, was successful and could be visualised by Confocal Laser Scanning Microscopy (CLSM). The C11-BODIPY(581/591) probe is a lipid oxidation reporter molecule, which is known to associate with the lipids of biological membranes and exhibit a fluorescence shift from the red range to the green range of the visible spectrum when it is oxidised together with the lipids. The present study is the first to demonstrate that the C11-BODIPY(581/591) probe can be used in dried membrane systems, and that a detection of oxidation is possible by CLSM analysis directly on the dried samples.
将荧光探针C11-硼二吡咯（581/591）掺入两种干燥膜系统中获得成功，这两种系统分别是在碳水化合物/蛋白质基质中冻干的大豆磷脂酰胆碱脂质体以及在碳水化合物基质中冻干的嗜酸乳杆菌（La-5），并且可以通过共聚焦激光扫描显微镜（CLSM）进行可视化观察。C11-硼二吡咯（581/591）探针是一种脂质氧化报告分子，已知它可与生物膜的脂质结合，并在与脂质一起被氧化时，其荧光从可见光谱的红色范围转变为绿色范围。本研究首次证明C11-硼二吡咯（581/591）探针可用于干燥膜系统，并且通过直接对干燥样品进行CLSM分析能够检测氧化情况。
