Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School & Hospital of Stomatology, Wuhan University, 237 Luoyu Road, 430079 Wuhan, Hubei, China.
Vaccine. 2009 Dec 9;27(52):7459-66. doi: 10.1016/j.vaccine.2009.05.007. Epub 2009 May 28.
As we know, the catalytic region of glucosyltransferases (GTFs) is a key region responsible for sucrose-dependent adherence of cariogenic bacteria to teeth. In this study, we evaluate the potential of the catalytic region to enhance the immunogenicity of the anti-caries DNA vaccine. We construct two new anti-caries DNA plasmids pGJGAC/VAX and pGJGA-5C/VAX by cloning different styles of the catalytic regions of GTFs into the previous plasmid pGJA-P/VAX. One is the 1.1 kb full length catalytic region of S. sobrinus GTF-I, the other is its catalytic core sequence which is conserved in the GTFs from mutans streptococci and plays a central role in the enzymatic activities of sucrose splitting and glucan synthesis. The results of caries protection experiment indicate that compared to pGJA-P/VAX, immunization with both new plasmids provides more effective protection against cariogenic bacteria, especially against S. sobrinus. The plasmid encoding full length catalytic region could provide more effective protection against cariogenic bacteria than that encoding catalytic core conserved sequence even though the differences are not very dramatic.
如我们所知，葡糖基转移酶（GTFs）的催化区域是负责致龋菌依赖蔗糖依附于牙齿的关键区域。在这项研究中，我们评估了催化区域增强抗龋 DNA 疫苗免疫原性的潜力。我们通过将 GTFs 的不同催化区域克隆到先前的质粒 pGJA-P/VAX 中，构建了两个新的抗龋 DNA 质粒 pGJGAC/VAX 和 pGJGA-5C/VAX。一个是 S. sobrinus GTF-I 的 1.1kb 全长催化区域，另一个是其催化核心序列，该序列在变异链球菌 GTFs 中保守，在蔗糖分解和葡聚糖合成的酶活性中发挥核心作用。龋齿保护实验的结果表明，与 pGJA-P/VAX 相比，用两种新质粒免疫可提供更有效的抗致龋菌保护，特别是对抗 S. sobrinus。尽管差异不是非常显著，但编码全长催化区域的质粒提供的抗致龋菌保护比编码催化核心保守序列的质粒更有效。
