Fröberg G Kastengren, Lindberg R, Ritter M, Nordlind K
Albano Animal Hospital, Danderyd, Sweden.
J Comp Pathol. 2009 Aug-Oct;141(2-3):89-97. doi: 10.1016/j.jcpa.2008.08.002. Epub 2009 May 15.
Mast cells of a number of different animal species have been reported to contain serotonin (5-hydroxytryptamine; 5-HT), a monoamine capable of numerous and complex actions, which may include an impact on tumour growth. Limited previous studies have suggested that normal or neoplastic canine mast cells do not express 5-HT. In the present study, canine cutaneous mast cell tumours (MCTs) of Patnaik histological grades I-III were investigated immunohistochemically for expression of 5-HT and its receptor (R) 5-HT1A. The proportion of positively labelled cells and the intensity of labelling of individual cells were determined. Both 5-HT and the 5-HT1A receptor were expressed by non-neoplastic dermal mast cells and neoplastic mast cells. More neoplastic mast cells expressed 5-HT than the 5-HT1AR. Poorly differentiated tumours expressed fewer of both molecules, but the better differentiated mast cells at the periphery of such lesions had more consistent 5-HT expression. 5-HT and the 5-HT1A receptor may be involved in the differentiation of canine MCTs and in the microvascular complications associated with these neoplasms.
据报道，许多不同动物物种的肥大细胞含有血清素（5-羟色胺；5-HT），这是一种能够产生多种复杂作用的单胺，其作用可能包括对肿瘤生长的影响。先前有限的研究表明，正常或肿瘤性犬肥大细胞不表达5-HT。在本研究中，对帕蒂克组织学分级为I-III级的犬皮肤肥大细胞瘤（MCT）进行免疫组织化学研究，以检测5-HT及其受体5-HT1A的表达。确定了阳性标记细胞的比例和单个细胞的标记强度。5-HT和5-HT1A受体均在非肿瘤性真皮肥大细胞和肿瘤性肥大细胞中表达。表达5-HT的肿瘤性肥大细胞比表达5-HT1A受体的更多。低分化肿瘤表达的这两种分子较少，但此类病变周边分化较好的肥大细胞5-HT表达更一致。5-HT和5-HT1A受体可能参与犬MCT的分化以及与这些肿瘤相关的微血管并发症。
